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Plasminogenuria is associated with podocyte injury, edema, and kidney dysfunction in incident glomerular disease

Egerman, Marc A; Wong, Jenny S; Runxia, Tian; Mosoyan, Gohar; Chauhan, Kinsuk; Reyes-Bahamonde, Joselyn; Anandakrishnan, Nanditha; Wong, Nicholas J; Bagiella, Emilia; Salem, Fadi; Meliambro, Kristin; Li, Hong; Azeloglu, Evren U; Coca, Steven G; Campbell, Kirk N; Raij, Leopoldo
Urinary plasminogen/plasmin, or plasmin (ogen) uria, has been demonstrated in proteinuric patients and exposure of cultured podocytes to plasminogen results in injury via oxidative stress pathways. A causative role for plasmin (ogen) as a "second hit" in kidney disease progression has yet to have been demonstrated in vivo. Additionally, association between plasmin (ogen) uria and kidney function in glomerular diseases remains unclear. We performed comparative studies in a puromycin aminonucleoside (PAN) nephropathy rat model treated with amiloride, an inhibitor of plasminogen activation, and measured changes in plasmin (ogen) uria. In a glomerular disease biorepository cohort (n = 128), we measured time-of-biopsy albuminuria, proteinuria, and plasmin (ogen) uria for correlations with kidney outcomes. In cultured human podocytes, plasminogen treatment was associated with decreased focal adhesion marker expression with rescue by amiloride. Increased glomerular plasmin (ogen) was found in PAN rats and focal segmental glomerulosclerosis (FSGS) patients. PAN nephropathy was associated with increases in plasmin (ogen) uria and proteinuria. Amiloride was protective against PAN-induced glomerular injury, reducing CD36 scavenger receptor expression and oxidative stress. In patients, we found associations between plasmin (ogen) uria and edema status as well as eGFR. Our study demonstrates a role for plasmin (ogen)-induced podocyte injury in the PAN nephropathy model, with amiloride having podocyte-protective properties. In one of the largest glomerular disease cohorts to study plasminogen, we validated previous findings while suggesting a potentially novel relationship between plasmin (ogen) uria and estimated glomerular filtration rate (eGFR). Together, these findings suggest a role for plasmin (ogen) in mediating glomerular injury and as a viable targetable biomarker for podocyte-sparing treatments.
PMID: 33070369
ISSN: 1530-6860
CID: 4641902

Complement in Non-Antibody-Mediated Kidney Diseases

Angeletti, Andrea; Reyes-Bahamonde, Joselyn; Cravedi, Paolo; Campbell, Kirk N
The complement system is part of the innate immune response that plays important roles in protecting the host from foreign pathogens. The complement components and relative fragment deposition have long been recognized to be strongly involved also in the pathogenesis of autoantibody-related kidney glomerulopathies, leading to direct glomerular injury and recruitment of infiltrating inflammation pathways. More recently, unregulated complement activation has been shown to be associated with progression of non-antibody-mediated kidney diseases, including focal segmental glomerulosclerosis, C3 glomerular disease, thrombotic microangiopathies, or general fibrosis generation in progressive chronic kidney diseases. Some of the specific mechanisms associated with complement activation in these diseases were recently clarified, showing a dominant role of alternative activation pathway. Over the last decade, a growing number of anticomplement agents have been developed, and some of them are being approved for clinical use or already in use. Therefore, anticomplement therapies represent a realistic choice of therapeutic approaches for complement-related diseases. Herein, we review the complement system activation, regulatory mechanisms, their involvement in non-antibody-mediated glomerular diseases, and the recent advances in complement-targeting agents as potential therapeutic strategies.
PMCID:5506082
PMID: 28748184
ISSN: 2296-858x
CID: 4551362

SERUM SODIUM RATE OF CHANGE AND VARIABILITY: ASSOCIATIONS WITH SURVIVAL IN INCIDENT HEMODIALYSIS PATIENTS [Meeting Abstract]

Reyes-Bahamonde, Joselyn; Raimann, Jochen G.; Canaud, Bernard; Etter, Michael; Kooman, Jeroen P.; Levin, Nathan W.; Marcelli, Daniele; Marelli, Cristina; Power, Albert; Van Der Sande, Frank M.; Thijssen, Stephan; Usvyat, Len A.; Wang, Yuedong; Kotanko, Peter
ISI:000338013500327
ISSN: 0931-0509
CID: 4551372

Fluid overload and inflammation--a vicious cycle

Reyes-Bahamonde, Joselyn; Raimann, Jochen G; Thijssen, Stephan; Levin, Nathan W; Kotanko, Peter
PMID: 23043638
ISSN: 1525-139x
CID: 4551352

RELATIONSHIP BETWEEN SERUM SODIUM VARIABILITY AND HOSPITAL ADMISSION IN HEMODIALYSIS PATIENTS [Meeting Abstract]

Reyes-Bahamonde, Joselyn; Raimann, Jochen; Usvyat, Len A.; Thijssen, Stephan; Van der Sande, Frank; Kooman, Jeroen; Levin, Nathan; Kotanko, Peter
ISI:000319498201311
ISSN: 0931-0509
CID: 4551382