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State of the viral DNA in rat cells transformed by polyoma virus. I. Virus rescue and the presence of nonintergrated viral DNA molecules

Prasad I; Zouzias D; Basilico C
The interaction of polyoma virus with a continuous line of rat cells was studied. Infection of these cells with polyoma did not cause virus multiplication but induced transformation. Transformed cells did not produce infectious virus, but in all clones tested virus was rescuable upon fusion with permissive mouse cells. Transformed rat cells contained, in addition to integrated viral genomes, 20 to 50 copies of nonintegrated viral DNA equivalents per cell (average). 'Free' viral DNA molecules were also found in cells transformed by the ts-a and ts-8 polyoma mutants and kept at 33 C. This was not due to a virus carrier state, since the number of nonintegrated viral DNA molecules was found to be unchanged when cells were grown in the presence of antipolyoma serum. Recloning of the transformed cell lines produced subclones, which also contained free viral DNA. Most of these molecules were supercoiled and were found in the muclei of the transformed cells. The nonintegrated viral DNA is infectious. Its specifici infectivity is, however, about 100-fold lower than that of polyoma DNA extracted from productively infected cells, suggesting that these molecules contain a large proportion of defectives
PMCID:515568
PMID: 178887
ISSN: 0022-538x
CID: 14475

Preparation of radioiodinated simian virus 40 DNA for use in D

Delap RJ; Zouzias D; Rush MG
A method is described for the preparation of 125I-labelled SV40 DNA. Using this method, SV40 DNA can be routinely labelled to 15 - 10(6) dpm per mug; much higher specific activities are easily obtained by minor modifications of the method. Once incorporated, the radioactive label dissociates from DNA exceedingly slowly at 4 degrees C or at 68 degrees C. Iodinated SV40 DNA is shown to be useful in the quantitation of viral nucleic acid sequences in SV40-transformed 3T3 cells by D
PMID: 174724
ISSN: 0006-3002
CID: 17083

REGULATION OF T-ANTIGEN AND VIRAL-RNA SYNTHESIS IN SV40- TRANSFORMED CELLS [Meeting Abstract]

Zouzias, DC; Basilico, C
ISI:A1976BR40301680
ISSN: 0014-9446
CID: 29475

STATE OF FREE VIRAL-DNA IN RAT CELLS TRANSFORMED BY POLYOMA- VIRUS [Meeting Abstract]

Prasad, I; Zouzias, D; Basilico, C
ISI:A1976BZ77400200
ISSN: 0021-9525
CID: 29449

Nucleic acid hybridization analysis of an integrated plasmid in Staphylococcus aureus

Novick R; Zouzias D; Rush M
A series of studies were performed on a Staphylococcus aureus strain thought to contain a pencillinase plasmid integrated into the host chromosome. Reassociation kinetics analysis of whole-cell deoxyribonucleic acid (DNA) in the presence of pure radioactive plasmid DNA revealed that plasmid-specific sequences were present at about 1 copy per chromosome equivalent as compared to 3.6 copies for the same plasmid in its autonomous state. Consistent with this observation was the finding that penicillinase activity was lower for the former strain than for the latter. It was shown further that the plasmid-specific sequences cosedimented on neutral sucrose gradients with fragments of whole-cell DNA many times larger than the plasmid. These two findings were taken as strongly confirmatory of the integrated state. Analysis of whole-cell ribonucleic acid for the presence of plasmid-specific messengers revealed that these were present in approximately the amounts expected on the basis of the DNA study
PMCID:236056
PMID: 1194240
ISSN: 0021-9193
CID: 17084

Studies on plasmid replication. II. In vivo transcription and its control in penicillinase plasmids from Staphylococcus aureus

Zouzias D; Rush M; Murphy E; Novick R
PMID: 4758063
ISSN: 0022-2836
CID: 17085

Extrachromosomal DNA of Mycoplasma hominis

Zouzias D; Mazaitis AJ; Simberkoff M; Rush M
PMID: 4125096
ISSN: 0006-3002
CID: 17086

Immunochemical studies on alpha crystallin subunits

Zouzias D; Manski W
PMID: 4624100
ISSN: 0014-4835
CID: 17087