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Identification and long term stability of DNA captured on a dental impression wafer

Kim, Maile; Siegler, Kate; Tamariz, Jeannie; Caragine, Theresa; Fernandez, Jill; Daronch, Marcia; Moursi, Amr
PURPOSE: The purpose of this study was to determine the quantity and quality of DNA extracted from a dental bite impression wafer immediately after impression and after 12 months of home storage. The authors' hypothesis was that the wafer would retain sufficient DNA with appropriate genetic markers to make an identification match. METHODS: Two impression wafers (Toothprints((R)) brand) were administered to 100 3- to 26-year-olds. A cotton swab was used as a control. DNA from wafers stored for 12 months at home were compared to DNA collected at time 0 and compared to swabs at specific sites to determine quality and accuracy. The amount of DNA captured and recovered was analyzed using MagAttract technology and a quantitative real-time polymerase chain reaction. Capillary gel electrophoresis was performed to determine the quality of the DNA profiles obtained from the wafers vs those generated from the swabs of each subject. RESULTS: Average DNA concentration was: 480 pg/muL (wafer at time 0); 392 pg/muL (wafer after 12 months kept by subjects); and 1,041 pg/muL (buccal swab). Sufficient DNA for human identification was recovered from all sets of wafers, producing clear DNA profiles and accurate matches to buccal swabs. No inhibitors were found that could interfere with DNA profiling. CONCLUSIONS: Toothprints(R) impression wafers can be useful for DNA collection and child identification. After 12 months, the wafer was still usable for DNA capture and identification match.
PMID: 23211911
ISSN: 0164-1263
CID: 271922

Interprofessional strategies to reduce the risk of early childhood caries [Meeting Abstract]

Hallas, D; Fernandez, J; Lim, L; Catapano, P
OBJECTIVES/SPECIFIC AIMS: To implement an interdisciplinary culturally sensitive oral health educational program for parents of newborns on the postpartum unit. METHODS/STUDY POPULATION: A prospective randomized controlled pretest, posttest experimental design was used to assess the effectiveness of an oral health education intervention for parents of newborns while on the postpartum unit. Ninety seven mothers and infants were enrolled. Each mother in the treatment and control group took the same pretest (instrument #1) prior to randomization. Twelve months later each mother took a posttest during the infant's first oral health examination. Instrument #2 was the Caries Risk Assessment and instrument #3 was the clinical oral health examination at the 12 month-old visit. RESULTS/ANTICIPATED RESULTS: Differences in the changes in the pretest and posttest scores will be determined by splitplot analysis of covariance (ANOVA). Mean scores on the caries risk assessment will be compared using the t-test. Findings from the oral health examinations from each infant in the treatment and control group will be assessed using the following scoring: no cavities = 0; white spots = 1; and cavitation = 2 (per tooth). Differences between the intervention group and the control group will be assessed by computing the chisquare statistic for the 2 x 3 cross tabulation table. All findings from the study including statistical analysis will be presented. DISCUSSION/SIGNIFICANCE OF IMPACT: An oral health educational program provided to mothers during the immediate post partum period has the potential to reduce the incidence of ECC in their infants
EMBASE:70803920
ISSN: 1752-8054
CID: 173071

Technology Enhanced Interprofessional Education for Dental and Ped iatric Nurse Practitioner Students

Chapter by: Fernandez, Jill B; Hallas, Donna
in: Clinical & Educational Scholarship Showcase by
[New York NY : NYU College of Dentistry. NYU Academy of Distinguished Educators], 2012
pp. 15-16
ISBN: n/a
CID: 350172

Use of mobile electronic devices as educational tool in pediatric community outreach

Fernandez, Jill B; Sadana, Chirag; Eisenberg, Elise S; Daronch, Marcia; Moursi, Amr M
The introduction of mobile electronic devices, as opposed to paper forms, in pediatric outreach programs of the New York University College of Dentistry is discussed. Since 2007, students have been receiving training on how to operate a personal digital assistant (PDA) and use it in community outreach for non-invasive oral-facial screenings and patient education. The shift from using paper forms to electronic media had a positive impact among the academic community, as it resulted in saving time and reducing the possibility of data collection errors. It may represent a significant improvement in data collection and patient education; and it provides an opportunity to enhance research and quality assessment.
PMID: 22338816
ISSN: 0028-7571
CID: 159249

Improving kids' oral health: Nearly 85% of children in Grenada suffer from untreated dental caries

Howard, Cynthia J.; Fernandez, Jill B.
Information about a two-week national oral health survey and outreach initiative in Grenada conducted by the New York University College of Dentistry (NYUCD) in January 2010 is presented. Professors Cynthia Howard and Jill Fernandez from NYUCD involved in the project share their experiences. Results of 1,075 dental exams performed on children between the ages of 6 and 15 revealed that nearly 85 percent of children in Grenada suffer from untreated dental caries
DOSS:67696997
ISSN: 0279-7720
CID: 273412

Nursing strategies to reduce the incidence of early childhood caries in culturally diverse populations

Hallas, Donna; Fernandez, Jill; Lim, Lily; Carobene, Macy
In the United States, early childhood caries (ECC) is a major unmet health care need adversely affecting the overall health of young children from diverse ethnic populations. Nurses who work in the newborn nursery, pediatrics, public, and community health centers have a unique opportunity to positively influence a change in this epidemic of ECC. Guided by Leininger's theory of cultural care, these authors describe ways to implement a comprehensive culturally sensitive oral health education program for parents of newborns and infants. Interventions based on the best available evidence for oral health education, a culturally sensitive caries risk assessment, recommendations for fluoride varnish treatments, and ways for parents to establish a dental home for the infant by 12 months old are presented.
PMID: 21601149
ISSN: 0882-5963
CID: 159240

Head Start In Clinical Dentistry -- Initial Clinical Experience

Chapter by: Larsen, Charlie; Fernandez, Jill; Mouris, Amir
in: Clinical & Educational Scholarship Showcase by
[New York NY : NYU College of Dentistry. NYU Academy of Distinguished Educators], 2011
pp. 17-18
ISBN: n/a
CID: 151827

Nutrition and oral health considerations in children with special health care needs: implications for oral health care providers

Moursi, Amr M; Fernandez, Jill B; Daronch, Marcia; Zee, Lena; Jones, Cassandra L
Children with special health care needs are at increased risk for oral diseases. The purpose of this article was to discuss: nutritional and oral health factors routinely observed in most chronic childhood disorders; dietary modifications associated with select systemic disorders and how they may impact oral health in children; and the following factors common to chronic disorders associated with diet modifications-decreased appetite and increased nutritional risk; frequency of food intake; parental overindulgence; long-term use of cariogenic medications; and xerostomia. Characteristics of childhood disorders that require dietary modifications (congenital heart disease, cystic fibrosis, cancer, AIDS/HIV, diabetes mellitus, and phenylketonuria) are summarized. In addition, healthy dietary modifications and oral health recommendations are suggested. Implementation of these recommendations can assist the dentist and dental team as they join physicians and nutritionists in delivering the best possible care to children with special health care needs
PMID: 20836954
ISSN: 0164-1263
CID: 155160

Public-private collaboration to improve oral health status of children enrolled in Head Start in New York City

Fernandez, Jill B; Herman, Neal G; Rosenberg, Linda R; Daronch, Marcia; Moursi, Amr M
A comprehensive oral health care program for Head Start children in New York City is described. Head Start is a federally funded pre-school program for low-income families and their children. It provides activities that help children grow mentally, socially, emotionally and physically. In 1994, a public-private partnership was created between New York Administration for Children's Services and New York University College of Dentistry. The program consists of periodic visits to different Head Start centers by a dental team composed of pediatric dentists, residents, hygienists and students. At the center, the team provides diagnostic and preventive services to children and oral health education to children, parents and staff. Referrals are then made to the College of Dentistry or to a community provider for treatment and follow-up. Free transportation is provided between Head Start centers and the college clinic. Over 13 years, 25,000 children have received diagnostic, preventive and treatment services
PMID: 18788178
ISSN: 0028-7571
CID: 153394

A unique role for dental school faculty: telephone triage training and integration into a health departments' emergency response planning

Fernandez, Jill B; Glotzer, David L; Triola, Marc M; Psoter, Walter J
OBJECTIVE: Dental professionals with proper training and integration into existing protocols for mobilization can be one additional resource during catastrophic events. A pilot project on training of dental school faculty in telephone triage in the event of an avian flu pandemic is described. A partnership was established with a grant from the Department of Justice/Department of Homeland Security, between the New York City Department of Health and Mental Hygiene, and New York University to initiate a pilot program to increase the manpower resources available to the health agency should an overwhelming public health event be present in the New York City area. METHODS: Eight faculties from New York University College of Dentistry were selected to receive telephone triage training consisting of 15 hours of formal presentations. This training was specifically designed to give participants a background in "outbreak investigations," and included a mock influenza outbreak. Also, a "phone triaging" training during a surge event was practiced. RESULTS: The training resulted in enabling alternative healthcare providers as capable personnel and one alternative source for a surge manpower pool. This was the innovative use of dental school faculty to bolster critically understaffed and overwhelmed areas in the NYCDOHMH infrastructure, such as call centers and for telephone triage, in their disaster scenarios, particularly in their response to avian flu. CONCLUSIONS: The established public health systems and medical community must understand the need to preplan for medical surge events and accept that a potential source of additional manpower could be the dental profession or other nontraditional healthcare personnel.
PMID: 18666510
ISSN: 1932-149x
CID: 156972