Faculty Bibliography

We have reexamined the retinal distribution and dendritic field dimensions of beta cells in the cat retina. Beta cells were labeled by retrograde transport from the A-layers of the lateral geniculate nucleus and distinguished from alpha cells on the basis of soma size. Dendritic fields of beta cells were visualized by intracellular staining in vitro. The fraction of cat ganglion cells that were beta cells varied with retinal location. Except near the area centralis, beta cells represented about half of all ganglion cells in the nasal hemiretina. They contributed as heavily as the other major ganglion cell classes to the nasal visual streak. In and near the area centralis and in the temporal retina, beta cells represented about two-thirds of all ganglion cells. The areas of beta cell dendritic fields were reciprocally related to beta cell density. For example, they were 3-fold smaller within the visual streak than at matched eccentricities outside it. For many cells, we could estimate both local beta cell density and dendritic field area. Coverage factor (dendritic field area x local density) remained constant at about 4 despite 100-fold variations in beta cell density, and was independent of eccentricity, nasotemporal location, or position relative to the visual streak. Analysis in terms of sampling theory suggests that the beta cell array is matched to X-cell spatial resolution so as to optimize acuity. The beta cell distribution and its systematic reflection in dendritic architecture predict acuity levels that apparently correlate well with actual visual performance across the cat's visual field

Ganglion cells of the cat retina that are neither alpha nor beta cells are often lumped for convenience into a single anatomical group--the gamma cells (Boycott & Wassle, 1974; Stone, 1983; Wassle & Boycott, 1991). Defined in this way, gamma cells are the morphological counterpart to the physiological W-cell class, which includes all ganglion cells that are neither Y (alpha) nor X (beta) cells. We have estimated the retinal distribution of gamma cells by using retrograde transport to label ganglion cells innervating the superior colliculus and by assuming that these included virtually all gamma cells and no beta cells. We excluded labeled alpha cells on the basis of soma size. Our data suggest that gamma cells represent just under half of the ganglion cells in most of the nasal retina, but only about a third of those in the area centralis and temporal retina. Gamma cells do not appear to be more highly concentrated in the nasal visual streak than are other ganglion cells. In the temporal retina, gamma cells with crossed projections to the brain are apparently at least twice as common as those with uncrossed projections

Sensory representations in the brain exhibit topographic variations in magnification. These variations have been thought to reflect regional differences in the density of innervation at the sensory receptor surface. In the primate visual cortex, for example, local magnification factors have been reported to be proportional to the corresponding densities of retinal ganglion cells. We sought to learn whether this principle also operates in a second major retinofugal pathway--the projection to the superior colliculus. In cats, we first used retrograde transport to determine the retinal distributions of the ganglion cells that project to the colliculus. Then, we compared the numbers of colliculopetal ganglion cells in selected retinal sectors to the areas of the corresponding collicular representations. Collicular areal magnification was not simply proportional to the density of afferent ganglion cells, being instead at least 5-fold greater than expected in the representation of the central visual field. These data imply that incoming retinal afferents are more widely spaced in the central regions of the tectal map than in the map's periphery. Such variations in afferent density appear to play as large a role as the distribution of ganglion cells in determining the metric of the collicular map