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Effect of ssbA1 and lexC113 mutations on lambda prophage induction, bacteriophage growth, and cell survival

Vales, L D; Chase, J W; Murphy, J B
Escherichia coli strains containing mutations (ssbA1 and lexC113) which affect single-strand deoxyribonucleic acid binding protein have been examined. Among the properties studied were: sensitivity to ultraviolet irradiation and methyl methane sulfonate, temperature sensitivity, induction of prophage lambda by ultraviolet light, temperature, and mitomycin C, and deoxyribonucleic acid synthesis. Strains containing the ssbA1 and lexC113 mutations differ significantly in several of these properties
PMCID:294387
PMID: 6451615
ISSN: 0021-9193
CID: 68514

Orientation of the guanine operon of Escherichia coli K-12 by utilizing strains containing guaB-xse and guaB-upp deletions

Vales, L D; Chase, J W; Murphy, J B
Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with respect to the xseA and upp genes. The order of the genes in this region is glyA-hisS-xseA-guaO-guaB-guaA-purG-upp-purC
PMCID:216865
PMID: 222730
ISSN: 0021-9193
CID: 68510