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18F-GE-180: a novel TSPO radiotracer compared to 11C-R-PK11195 in a preclinical model of stroke
Boutin, Hervé; Murray, Katie; Pradillo, Jesus; Maroy, Renaud; Smigova, Alison; Gerhard, Alexander; Jones, Paul A; Trigg, William
PURPOSE/OBJECTIVE:Neuroinflammation plays a critical role in various neuropathological conditions, and hence there is renewed interest in the translocator protein (TSPO) as a biomarker of microglial activation and macrophage infiltration in the brain. This is reflected in the large amount of research conducted seeking to replace the prototypical PET radiotracer (11)C-R-PK11195 with a TSPO ligand with higher performance. Here we report the in vivo preclinical investigation of the novel TSPO tracer (18)F-GE-180 in a rat model of stroke. METHODS:Focal cerebral ischaemia was induced in Wistar rats by 60-min occlusion of the middle cerebral artery (MCAO). Brain damage was assessed 24 h after MCAO by T2 MRI. Rats were scanned with (11)C-R-PK11195 and (18)F-GE-180 5 or 6 days after MCAO. Specificity of binding was confirmed by injection of unlabelled R-PK11195 or GE-180 20 min after injection of (18)F-GE-180. In vivo data were confirmed by ex vivo immunohistochemistry for microglial (CD11b) and astrocytic biomarkers (GFAP). RESULTS:(18)F-GE-180 uptake was 24 % higher in the core of the ischaemic lesion and 18 % lower in the contralateral healthy tissue than that of (11)C-R-PK11195 uptake (1.5 ± 0.2-fold higher signal to noise ratio). We confirmed this finding using the simplified reference tissue model (BPND = 3.5 ± 0.4 and 2.4 ± 0.5 for (18)F-GE-180 and (11)C-R-PK11195, respectively, with R 1 = 1). Injection of unlabelled R-PK11195 or GE-180 20 min after injection of (18)F-GE-180 significantly displaced (18)F-GE-180 (69 ± 5 % and 63 ± 4 %, respectively). Specificity of the binding was also confirmed by in vitro autoradiography, and the location and presence of activated microglia and infiltrated macrophages were confirmed by immunohistochemistry. CONCLUSION/CONCLUSIONS:The in vivo binding characteristics of (18)F-GE-180 demonstrate a better signal to noise ratio than (11)C-R-PK11195 due to both a better signal in the lesion and lower nonspecific binding in healthy tissue. These results provide evidence that (18)F-GE-180 is a strong candidate to replace (11)C-R-PK11195.
PMID: 25351507
ISSN: 1619-7089
CID: 5355042
Metastatic papillary renal cell carcinoma regression after cytoreductive nephrectomy [Case Report]
Williams, Travis; Rodriguez, Robert; Murray, Katie; Kovaleski, Aaron; Madan, Rashna; Van Veldhuizen, Peter
PMID: 25623665
ISSN: 1527-9995
CID: 5355062
ENDOSCOPIC MANAGEMENT FOR UPPER TRACT UROTHELIAL CANCER (UTUC) COMPARED TO IMMEDIATE NEPHROURETERECTOMY: SURVIVAL OUTCOMES IN SEER DATABASE AND CANCER CENTER COHORT [Meeting Abstract]
Murray, Katie; Winer, Andrew; Bagrodia, Aditya; Kaffenberger, Samuel; Vacchio, Michael; Sankin, Alexander; Cha, Eugene; Benfante, Nicole; Dalbagni, Guido; Vetter, Joel; Coleman, Jonathan; Strope, Seth
ISI:000362552200151
ISSN: 0022-5347
CID: 5355582
HISTOLOGICAL VARIANTS OF UPPER URINARY TRACT UROTHELIAL CARCINOMA: THE 18-YEAR MSKCC EXPERIENCE [Meeting Abstract]
Winer, Andrew; Vacchio, Michael; Murray, Katie; Kaffenberger, Samuel; Bagrodia, Aditya; Cha, Eugene; Sankin, Alexander; Sfakianos, John; Dalbagni, Guido; Coleman, Jonathan
ISI:000362552200161
ISSN: 0022-5347
CID: 5355592
FOLLOW-UP SURGICAL INTERVENTIONS IN PATIENTS WITH URINARY DIVERSION: A COMPARISON BETWEEN ORTHOTOPIC NEOBLADDERS AND ILEAL CONDUITS [Meeting Abstract]
Flores, David; Murray, Katie; Parker, William; Zainfeld, Daniel; Mirza, Moben; Holzbeierlein, Jeffrey
ISI:000362552200574
ISSN: 0022-5347
CID: 5355602
ROBOTIC-ASSISTED EPIGASTRIC ARTERY HARVEST: A NOVEL APPROACH TO PENILE REVASCULARIZATION [Meeting Abstract]
Aberger, Michael; Murray, Katie; Broghammer, Joshua; Duchene, David
ISI:000362826300144
ISSN: 0022-5347
CID: 5355612
MULTIPLE PRIMARY MALIGNANCIES IN RENAL CORTICAL NEOPLASMS: AN UPDATED EVALUATION [Meeting Abstract]
Murray, Katie S.; Zabor, Emily C.; Spaliviero, Massimiliano; Russo, Paul; Bazzi, Wassim M.; Musser, John E.; Hakimi, A. Ari; Bernstein, Melanie L.; Dalbagni, Guido; Coleman, Jonathan A.; Furberg, Helena
ISI:000362826500154
ISSN: 0022-5347
CID: 5355622
TREATMENT OF THE PRIMARY TUMOR WITH VASCULAR-TARGETED PHOTODYNAMIC THERAPY (VTP) OVERCOMES RESISTANCE TO SYSTEMIC PD-1/PD-L1 INHIBITION: EFFECTS ON PRIMARY TUMOR CONTROL AND PREVENTION OF LUNG METASTASIS IN A PRE-CLINICAL RCC MODEL [Meeting Abstract]
O\Shaughnessy, Matthew; Murray, Katie; LaRosa, Stephen; Monette, Sebastien; Kim, Kwanghee; Beluco, Renato; Fonseca, Corradi; Scherz, Avigdor; Coleman, Jonathan
ISI:000362826500376
ISSN: 0022-5347
CID: 5355632
PHASE 3 RANDOMIZED TRIAL OF INTRAVENOUS MANNITOL VERSUS PLACEBO PRIOR TO RENAL ISCHEMIA DURING PARTIAL NEPHRECTOMY: IMPACT ON RENAL FUNCTIONAL OUTCOMES [Meeting Abstract]
Spaliviero, Massimiliano; Power, Nicholas E.; Murray, Katie S.; Russo, Paul; Sjoberg, Daniel D.; Benfante, Nicole E.; Sun, Arony J.; Bernstein, Melanie L.; Touijer, Karim A.; Dalbagni, Guido; Coleman, Jonathan A.
ISI:000362826500570
ISSN: 0022-5347
CID: 5355642
IRREVERSIBLE ELECTROPORATION (IRE) AS A LOCALIZED TREATMENT FOR PROSTATE CANCER: A REPORT ON SAFETY AND OUTCOMES [Meeting Abstract]
Murray, Katie; Musser, John; Mashni, Joseph; Srimathveeravalli, Govindarajan; Durack, Jeremy; Solomon, Stephen; Coleman, Jonathan
ISI:000362826600293
ISSN: 0022-5347
CID: 5355652