Faculty Bibliography

The effects of chrysene and the ozonated byproducts on in vitro gap junctional intercellular communication (GJIC) were evaluated using the scrape loading/dye transfer (SL/DT) technique. A 1 mM solution of chrysene was ozonated at dosages of 1.75, 3, 4.25, and 5 mol O3/mol chrysene (Chr). The early ozonation mixture, 1.75 mol O3/mol Chr, exhibited greater inhibition to GJIC than chrysene and irreversible damage to cells leading to cell death. To determine the compounds potentially responsible for the increase in toxicity, the byproducts formed upon treatment with 1.44 mol O3/mol Chr were separated into 14 fractions using RP-HPLC. The major compounds identified in the fractions were 2-(2'-formyl) phenyl-1-naphthaldehyde, 2-(2'formyl) phenyl-1-naphthoic acid, and 2-2-carboxyphenyl-1-naphthoic acid. 2-(2'-Formyl) phenyl-1-naphthaldehyde was determined to be the compound causing GJIC inhibition in sample fractions and byproduct mixtures.

AIM/OBJECTIVE:To study the reversal effects of droloxifene (DRO) on multidrug resistance (MDR) in K562 cell line resistant to adriamycin (ADR). METHODS:K562 cell line resistant to ADR (K562/A02) and K562 cell line sensitive to ADR (K562) were treated with DRO. Using MTT assay, chemosensitivity to ADR in DRO-treated K562 cell lines was studied. Before and after the treatment with DRO 10 micromol/L, MDR1 and GSTpi gene expression were assayed by reverse transcription-polymerase chain reaction and immunocytochemistry assay. Flow cytometry was used to determine intracellular ADR concentration. RESULTS:DRO significantly reversed MDR in K562/A02 (P < 0.01). After treatment of DRO 20, 10, and 5 micromol/L, the chemosensitivity to ADR was increased to 14, 13, and 4 folds, respectively. The reversal activity of DRO was similar to that of verapamil (VRP). After treated with DRO 10 micromol/L, both MDR1 and GSTpi mRNA expression began to decline on the 2nd day, and significantly decreased on the 5th day (P<0.01). The changes in P-gp and GSTpi protein expression were similar to that of their mRNA expression. Two hours after treatment of DRO 20, 10, and 5 micromol/L, intracellular ADR concentration in K562/A02 was increased to 2.9, 2.3, and 1.5 folds, respectively. However, DRO did not markedly increase ADR accumulation in K562. CONCLUSION/CONCLUSIONS:DRO had strong reversal effect on MDR in K562/A02, which was comparable to that of VRP, but the reversal effect was via different pathways.

Cerebral spinal fluid (CSF) pulse waveform basing on 899 records was classified. Eleven types of CSF pulse waveform were further classified according to the harmonic wave relation and the configuration of the pulse waveform. The characteristic features of various types of CSF pulse waveform were considered to reflect certain intracranial pathophysiological conditions including cerebral compliance, cerebral vasomotor reactivity and intracranial elastance.

The results of the Be-induced leukocyte migration inhibition test (Be-MIT), the lymphocyte transformation test (LI), the E-rosette formation test (E-RFC), the old tuberculin test (OT), and sero-immunoglobulin (IgG, IgA, IgM) measurements on 13 cases of berylliosis, 44 members of the observation group, and 51 members of the contact group are reported. In the Be-MIT, 2 of the berylliosis patients were new cases that had been diagnosed and treated for a long time, and both tested positive (18%). Fourteen patients from the observation group tested positive (32%), and 15 from the contact group tested positive (30%). The 8 cases in the control group tested negative; 7 patients from the observation group and 3 of the berylliosis cases tested negative after being treated. The lymphocyte transformation rates of the observation group and the berylliosis group were significantly lower than that of the control group (P less than 0.01). Both Et-RFC and Ea-RFC contents in the group with berylliosis were much lower than those in the control group (P less than 0.01). OT tests were performed on 8 berylliosis patients and 10 healthy men. Seven patients showed negative reactions and one showed a positive reaction. All the healthy men showed positive reactions. The IgG and IgA levels of the observation group were significantly higher than those of the control group (P less than 0.01). The Be-MIT could detect active beryllium disease at an early stage as well as Be-sensitive individuals. This test could be used as a supplementary diagnostic index for the differential diagnosis of berylliosis and for the evaluation of a curative effect.

beta-endorphin-like immunoreactivity (beta-ELI) was measured in cerebrospinal fluid (CSF) of 36 acute head-injured patients and 12 patients without head injury as controls. The mean level of beta-ELI in CSF of controls, mild cerebral contusions, and severe cerebral contusion patients were 51.9 +/- 5.6 pg/ml, 110.5 +/- 14.5 pg/ml, and 173.8 +/- 20.1 pg/ml respectively, with significant difference between them. The results also showed that beta-ELI may reflect the prognosis of acute head-injured patients.