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The inhibitory effect of BSP-A1/-A2 on protein kinase C and tyrosine protein kinase
Yu, Bingzhi; Zhao, Yongmei; Zhao, Wei; Chen, Fei; Liu, Ying; Zhang, Jie; Fu, Wei; Zong, Zhihong; Yu, Aiming; Guan, Yi
Bovine seminal plasma contains a group of similar proteins, namely BSP-A1, BSP-A2, BSP-A3, and BSP-30-kDa (collectively called BSP proteins), and they are secreted by the seminal vesicles. In our study, we purified the BSP-A1/-A2 through affinity chromatography and found for the first time that BSP-A1/-A2 can inhibit the activity of protein kinase C (PKC) and tyrosine protein kinase (TPK). The inhibition was dose dependent. When the PKC and TPK activities are expressed as the logarithm of percentage activity taking the activity in the absence of the BSP-A1/-A2 as 100%, there is a linear relationship between the their activities and the dose of BSP-A1/-A2.
PMID: 12736909
ISSN: 0263-6484
CID: 5477062
Effect of PKC-zeta on the regulation of genome transcription of the early development of fertilized eggs in the mouse
Fu, W; Zao, Y; Yu, A; Liu, Y; Zong, Z; Chen, Fei; Yu, B
ORIGINAL:0016790
ISSN: 1007-7626
CID: 5477152
Insulin and phorbol ester activate p70S6k through different pathway during protein synthesis
Zhao, Y; Fu, W; Song, Y; Wang, Y; Liu, Y; Chen, Fei; Zong, Z; Han, Y; Yu, B
ORIGINAL:0016789
ISSN: 1007-7626
CID: 5477142
The activity and expression of protein kinase B in the early development of mouse fertilized eggs
Chen, Fei; Yu, A; Feng, C; Fu, W; Zhao, Y; Yuan, Y; Wu, D; Yu, B
ORIGINAL:0016788
ISSN: 1007-7626
CID: 5477132
The effect of protamine on the transcription of one-cell sate fertilized eggs in the mouse
Yu, A; Chen, Fei; Wu, D; Zong, Z; Yu, B
ORIGINAL:0016792
ISSN: 1007-7626
CID: 5477172
Different expression of PKC-delta and PKC-theta in testes of mouse at various developmental stages
Feng, C; Fu, W; Zhao, Y; Chen, Fei; Zong, Z
ORIGINAL:0016791
ISSN: 1007-7626
CID: 5477162
Gene polymorphism related to the wisdom teeth in Han families
Liu, Y; Yan, X; Chen, Fei; Yu, B
ORIGINAL:0016786
ISSN: 1001-6007
CID: 5477112
Relationship between serum soluble E-selectin and type 2 diabetes millitus and its associated microangiopathy
Zhang, J; Chen, Fei; Ma, B
ORIGINAL:0016803
ISSN: 1000-5161
CID: 5479512
Purification of bovine seminal plasma (BSP) protein from bovine seminal plasma by affinity chromatography
Zhao, W; Chen, Fei; Liu, Q; Yu, B
ORIGINAL:0016787
ISSN: 1001-6007
CID: 5477122