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Smoke-Associated Microbiome Exposure Leads to Alteration of Inflammation that Impacts Emphysema Development [Meeting Abstract]
Wu, B.; Xiao, R.; Perez, L.; Franca, B.; Wang, A.; Carpenito, J.; Blazoski, C.; Olsen, E.; Zelonina, T.; Li, Y.; Blaser, M. J.; D'Armiento, J. M.; Segal, L. N.
ISI:000449980305184
ISSN: 1073-449x
CID: 3512842
The Mycobacteriome: A Nested Approach to Identify Non-Tuberculous Mycobacterium [Meeting Abstract]
Sulaiman, I.; Wu, B.; Scaglione, B. D.; Wang, J.; Basavaraj, A.; Li, Y.; Scott, A. S.; Chang, S.; Bantis, K.; Clemente, J.; Bessich, J. L.; Rafeq, S.; Michaud, G. C.; Donington, J. S.; Naidoo, C.; Theron, G.; Condos, R.; Kamelhar, D.; Addrizzo-Harris, D. J.; Segal, L. N.
ISI:000449978902397
ISSN: 1073-449x
CID: 3513362
Single Cell RNA Sequencing Profiling of Pulmonary and Systemic T Cells in Subjects with Lung Cancer [Meeting Abstract]
Beattie, J.; Sulaiman, I.; Wu, B.; Li, Y.; Franca, B.; Perez, L.; Tsay, J. J.; Segal, L. N.
ISI:000449980302393
ISSN: 1073-449x
CID: 3513012
The Microbiota of Non-Tuberculosis Mycobacterium Leads to a Distinct Inflammatory Profile [Meeting Abstract]
Sulaiman, I.; Wu, B.; Scaglione, B. D.; Wang, J.; Basavaraj, A.; Li, Y.; Scott, A. S.; Chung, S.; Bantis, K.; Clemente, J.; Shen, N.; Bessich, J. L.; Rafeq, S.; Michaud, G. C.; Donington, J. S.; Naidoo, C.; Theron, G.; Condos, R.; Kamelhar, D.; Addrizzo-Harris, D. J.; Segal, L. N.
ISI:000449978905391
ISSN: 1073-449x
CID: 3513172
Lung microbiome and host immune tone in subjects with idiopathic pulmonary fibrosis treated with inhaled interferon-gamma
Wang, Jing; Lesko, Melissa; Badri, Michelle H; Kapoor, Bianca C; Wu, Benjamin G; Li, Yonghua; Smaldone, Gerald C; Bonneau, Richard; Kurtz, Zachary D; Condos, Rany; Segal, Leopoldo N
Therapies targeting inflammation reveal inconsistent results in idiopathic pulmonary fibrosis (IPF). Aerosolised interferon (IFN)-gamma has been proposed as a novel therapy. Changes in the host airway microbiome are associated with the inflammatory milieu and may be associated with disease progression. Here, we evaluate whether treatment with aerosolised IFN-gamma in IPF impacts either the lower airway microbiome or the host immune phenotype. Patients with IPF who enrolled in an aerosolised IFN-gamma trial underwent bronchoscopy at baseline and after 6 months. 16S rRNA sequencing of bronchoalveolar lavage fluid (BALF) was used to evaluate the lung microbiome. Biomarkers were measured by Luminex assay in plasma, BALF and BAL cell supernatant. The compPLS framework was used to evaluate associations between taxa and biomarkers. IFN-gamma treatment did not change alpha or beta diversity of the lung microbiome and few taxonomic changes occurred. While none of the biomarkers changed in plasma, there was an increase in IFN-gamma and a decrease in Fit-3 ligand, IFN-alpha2 and interleukin-5 in BAL cell supernatant, and a decrease in tumour necrosis factor-beta in BALF. Multiple correlations between microbial taxa common to the oral mucosa and host inflammatory biomarkers were found. These data suggest that the lung microbiome is independently associated with the host immune tone and may have a potential mechanistic role in IPF.
PMCID:5507144
PMID: 28717640
ISSN: 2312-0541
CID: 2639962
Lung Microbiota and Its Impact on the Mucosal Immune Phenotype
Wu, Benjamin G; Segal, Leopoldo N
The use of culture-independent techniques has allowed us to appreciate that the upper and lower respiratory tract contain a diverse community of microbes in health and disease. Research has only recently explored the effects of the microbiome on the host immune response. The exposure of the human body to the bacterial environment is an important factor for immunological development; thus, the interaction between the microbiome and its host is critical to understanding the pathogenesis of disease. In this article, we discuss the mechanisms that determine the composition of the airway microbiome and its effects on the host immune response. With the use of ecological principles, we have learned how the lower airways constitute a unique niche subjected to frequent microbial migration (e.g., through aspiration) and constant immunological pressure. The discussion will focus on the possible inflammatory pathways that are up- and downregulated when the immune system is challenged by dysbiosis. Identification of potential markers and microbial targets to address the modulation of inflammation in early disease, when changes may have the most effect, will be critical for future therapies.
PMCID:5484071
PMID: 28643622
ISSN: 2165-0497
CID: 4256572
Anaerobic Bacterial Fermentation Products Increase Tuberculosis Risk in Antiretroviral-Drug-Treated HIV Patients
Segal, Leopoldo N; Clemente, Jose C; Li, Yonghua; Ruan, Chunhai; Cao, Jane; Danckers, Mauricio; Morris, Alison; Tapyrik, Sarah; Wu, Benjamin G; Diaz, Philip; Calligaro, Gregory; Dawson, Rodney; van Zyl-Smit, Richard N; Dheda, Keertan; Rom, William N; Weiden, Michael D
Despite the immune-reconstitution with antiretroviral therapy (ART), HIV-infected individuals remain highly susceptible to tuberculosis (TB) and have an enrichment of oral anaerobes in the lung. Products of bacterial anaerobic metabolism, like butyrate and other short-chain fatty acids (SCFAs), induce regulatory T cells (Tregs). We tested whether SCFAs contribute to poor TB control in a longitudinal cohort of ART-treated HIV-infected South Africans. Increase in serum SCFAs was associated with increased TB susceptibility. SCFAs inhibited IFN-gamma and IL-17A production in peripheral blood mononuclear cells from HIV-infected ART-treated individuals in response to M. tuberculosis antigen stimulation. Pulmonary SCFAs correlated with increased oral anaerobes, such as Prevotella in the lung, and with M. tuberculosis antigen-induced Tregs. Metabolites from anaerobic bacterial fermentation may, therefore, increase TB susceptibility by suppressing IFN-gamma and IL-17A production during the cellular immune response to M. tuberculosis.
PMCID:5465639
PMID: 28366509
ISSN: 1934-6069
CID: 2545332
Airway Microbiota Shifts During Stable Cystic Fibrosis Treated With Inhaled Antibiotics Are Associated With Exacerbations And Disease Progression [Meeting Abstract]
Sulaiman, I; Beatty, J; Scaglione, B; Wu, BG; Wang, J; Scott, AS; Giusti, R; Amoroso, N; DiMango, E; Fiel, SB; Berdella, M; Walker, P; Condos, R; Segal, LN
ISI:000400372507409
ISSN: 1535-4970
CID: 2591342
The Airway Dna Virome In Healthy Smokers And Nonsmokers [Meeting Abstract]
Keller, BC; Gregory, A; Zhao, G; Wu, BG; Sullivan, M; Clemente, J; Segal, LN
ISI:000400372506016
ISSN: 1535-4970
CID: 2591282
Lung Cancer And Lung Microbiome [Meeting Abstract]
Tsay, JJ; Clemente, J; Lhakhang, T; Li, Y; Yie, T-A; Wu, BG; Kapoor, B; Wang, J; Sterman, DH; Heguy, A; Rom, WN; Blaser, M; Segal, LN
ISI:000400372500002
ISSN: 1535-4970
CID: 2591562