Faculty Bibliography

Normal maternal thyroid function during pregnancy is essential for fetal development and depends upon an adequate supply of iodine. Little is known about how iodine status is associated with preterm birth and small for gestational age (SGA) in mildly iodine insufficient populations. Our objective was to evaluate associations of early pregnancy serum iodine, thyroglobulin (Tg), and thyroid-stimulating hormone (TSH) with odds of preterm birth and SGA in a prospective, population-based, nested case-control study from all births in Finland (2012-2013). Cases of preterm birth (n = 208) and SGA (n = 209) were randomly chosen from among all singleton births. Controls were randomly chosen from among singleton births that were not preterm (n = 242) or SGA (n = 241) infants during the same time period. Women provided blood samples at 10-14 weeks' gestation for serum iodide, Tg and TSH measurement. We used logistic regression to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for preterm birth and SGA. Each log-unit increase in serum iodide was associated with higher odds of preterm birth (adjusted OR = 1.19, 95% CI = 1.02-1.40), but was not associated with SGA (adjusted OR = 1.01, 95% CI = 0.86-1.18). Tg was not associated with preterm birth (OR per 1 log-unit increase = 0.87, 95% CI = 0.73-1.05), but was inversely associated with SGA (OR per log-unit increase = 0.78, 95% CI = 0.65-0.94). Neither high nor low TSH (versus normal) were associated with either outcome. These findings suggest that among Finnish women, iodine status is not related to SGA, but higher serum iodide may be positively associated with preterm birth.

The goal of this study was to assess biomarkers of exposure to glyphosate and assess potential associations with renal function in children. Glyphosate is used ubiquitously in agriculture worldwide. While previous studies have indicated that glyphosate may have nephrotoxic effects, few have examined potential effects on kidney function in children. We leveraged three cohorts across different phases of child development and measured urinary levels of glyphosate. We evaluated associations of glyphosate with three biomarkers of kidney injury: albuminuria (ACR), neutrophil gelatinase-associated lipocalin (NGAL), and kidney injury marker 1 (KIM-1). Multivariable regression analyses examined associations of glyphosate with kidney injury biomarkers controlling for covariates. We identified glyphosate in 11.1% of the total participants. The herbicide was detected more frequently in the neonate population (30%). Multivariable regression models failed to identify significant associations of log-transformed glyphosate with any of the kidney injury biomarkers, controlling for covariates age, sex, and maternal education. While we confirm detectability of glyphosate in children's urine at various ages and stages of life, there is no evidence in this study for renal injury in children exposed to low levels of glyphosate. Further studies of larger sample size are indicated to better understand putative deleterious effects of the herbicide after different levels of exposure.

Human exposure to microplastics has been a topic of interest, but measurements of exposure are limited. Pet animals are sentinels of human exposure, as they share a common living environment with humans. In this study, 58 pet (cat and dog) foods and 78 pet feces samples were collected from Albany, NY, USA, for the analysis of polyethylene terephthalate (PET) and polycarbonate (PC) by alkali-assisted thermal depolymerization and liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) methods. PET was detected at concentrations in the range of <1,500 ng/g to 12,000 ng/g (median: <1,500 ng/g) and <1,500 to 4,600 ng/g (median: <1,500 ng/g) in cat and dog foods, respectively. The concentrations of PET in cat (<2,300-340,000 ng/g, median: 61,000 ng/g) and dog (7700-190,000 ng/g, median: 30,000 ng/g) feces were 1-2 orders of magnitude higher than those in pet food samples. A significant positive correlation was found between the concentrations of the monomers (i.e., TPA and BPA) and the corresponding MPs in cat feces. The calculated mean estimated daily intake of PET and PC (calculated from pet food) was lower than that of the mean cumulative daily intake (calculated from pet feces), which suggested that diet is a minor source of exposure to PET and PC in pets.

Human exposure to endocrine-disrupting chemicals (EDCs) has aroused considerable public concern over the last three decades. Nevertheless, little is known with regard to the exposure of EDCs in farm animals. In this study, concentrations of 22 phthalate metabolites (PhMs), 15 hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs), and 8 bisphenols (BPs) were determined in 183 bovine urine samples collected from China, India, and the United States. The median concentrations of urinary PhMs, OH-PAHs, and BPs in bovines, collectively, were 66, 4.6, and 16 ng/mL, respectively. Mono-n-butyl phthalate (mBP; median, 14 ng/mL) and ∑₄DEHP (four secondary metabolites of di(2-ethylhexyl) phthalate; 13 ng/mL) were the dominant PhMs; hydroxy-fluorene (OH-Fluo; 1.2 ng/mL) and -phenanthrene (OH-Phen; 1 ng/mL) were the dominant OH-PAHs; and 4,4'-di-hydroxydiphenylmethane (BPF; 10 ng/mL) and 2,2-bis(4-hydroxyphenyl) propane (BPA; 6.7 ng/mL) were the dominant BPs. Bovine urine samples from India and China contained the highest concentrations of PhMs and OH-PAHs, whereas those from India and the United States contained the highest concentrations of BPs. PhM and OH-PAH concentrations were significantly higher in the urine of bulls than those of cows; no such difference was found for BPs. Our findings establish baseline exposure information about three classes of EDCs in domestic farm animals.

Despite high production and usage of parabens and bisphenols, little is known about their spatiotemporal distribution in the marine environment. In this study, we determined the concentrations of several parabens and their metabolites as well as bisphenol analogues in sediment collected from coastal areas of northern China. All sediment samples, including surface sediment and sediment cores, contained at least one of the parabens analyzed, and the total concentrations of parabens (ΣPBs; sum of six parabens) ranged from 1.37 to 24.2 ng/g dw (geometric mean: 3.30-6.09 g/g dw), which was comparable to or slightly higher than those found for the total concentrations of five detectable bisphenols (ΣBPAs; geometric mean: 2.18-4.61 ng/g dw). 4-hydroxybenzoic acid, a common metabolite of parabens, was found in all samples at concentrations in the range of 6.85-437 ng/g dw, which was one order of magnitude lower than those found for benzoic acid. Methyl-, ethyl-, and propyl-parabens were the predominant paraben analogues, collectively accounting for >88% of ΣPBs. Bisphenol A and bisphenol F were the two major bisphenols, collectively accounting for >86% of ΣBPAs. We also examined vertical profiles in concentrations of target analytes in sediment cores. The sediment core from the Shandong Peninsula showed a gradual increase in the concentrations of several parent and metabolic parabens as well as bisphenols during the past decade. Relatively higher concentrations of parabens and bisphenols were found in sediment cores collected from industrialized areas. Significant positive correlations were found among the concentrations of parabens in sediment, which suggested the existence of similar sources for these compounds. Overall, our findings suggest that the Bohai Sea coast is moderately contaminated with parabens and bisphenols in comparison to other coastal areas in China or elsewhere.

Organophosphate esters (OPEs) are used in aircraft lubricating oil and hydraulic fluids, and, thus, airplane emissions are thought to be an important source of these chemicals in the environment. In this study, concentrations of 20 OPEs, comprising seven alkyl-OPEs, three chlorinated (Cl)-OPEs, seven aryl-OPEs, and three oligomeric-OPEs, were determined in outdoor air, soil, pine needles, river water, and outdoor dust samples collected around an airport in Albany, New York, in 2018. Elevated ∑OPE concentrations were found in outdoor air, soil, pine needles, outdoor dust, and river water in the ranges of 1320-20,700 pg/m³ (median: 3880), 1.16-73.1 (14.3) ng/g dry weight (dw), 23.2-534 (102) ng/g (dw), 153-2140 (824) ng/g (dw), and 174-24,600 (1250) ng/L, respectively. The total OPE concentrations in air, soil, water, and outdoor dust samples in the study area were dominated by Cl-OPEs, whereas those in pine needles were dominated by aryl-OPEs. The spatial distribution of OPEs in air, soil, and pine needles showed a gradual decreasing trend with increasing distance from the airport. A significant correlation was observed between ∑OPE concentrations in air and soil, and the fugacity ratio showed the flux of OPEs from air to soil. The spatial distribution of OPEs between air and pine needles was similar and highly correlated, suggesting that pine needles are suitable indicators of atmospheric OPE concentrations. In addition to urban activities, aircraft hydraulic/lubricant oils are a major source of OPEs in the vicinity of the airport. The average daily intake of OPEs via air inhalation and outdoor dust ingestion in the vicinity of the airport was up to 1.53 ng/kg bw/day for children and 0.73 ng/kg bw/day for adults.

Wastewater, aeration gas, dewatered sludge, and incineration ash and flue gas (from dewatered sludge) were collected from 9 sewage treatment plants (STPs) located in Saitama Prefecture, Japan, and analyzed for seven cyclic and linear volatile methylsiloxanes (VMSs) namely, D3, D4, D5, D6, L3, L4, and L5. The mass loadings and distribution of VMSs in STPs were estimated based on measured concentrations in liquid, solid, and gaseous samples, including incinerated dewatered sludge. Mass loading of ΣVMS varied widely from 21 kg y^-1 to 3740 kg y^-1, depending on the volume of wastewater treated in each STP. Mass % of ΣVMS distributed in aeration gas was 15% and that in activated sludge was 78%. Approximately 6.6% of ΣVMS remained in the final effluent. Overall, partitioning onto the activated sludge was the dominant removal mechanism for D4, D5, and D6, whereas volatilization was also an important removal mechanism for D4. Incineration was effective to degrade VMSs in dewatered sludge, with a reduction rate of >99%. Activated carbon treatment removed >99% of VMSs from the aeration gas. In Saitama Prefecture, total emission of ΣVMS via STPs was estimated at 434 kg y^-1, 86 kg y^-1, and 0.065 kg y^-1, to aquatic, atmospheric, and terrestrial environments, respectively, which accounted for 83%, 17%, and 0.01% of the total environmental emissions. Our results indicate that majority of VMSs in dewatered sludge can be removed by incineration and emission of VMSs through incineration ash landfill is negligible.

Organophosphate esters (OPEs) are used in consumer products as flame retardants and plasticizers. Little is known, however, about the occurrence and profiles of OPEs in human milk. In this study, 14 OPEs were measured in 100 breast milk samples collected from the United States during the period of 2009-2012, using high-performance liquid chromatography and tandem mass spectrometry. The sum concentrations of 14 OPEs in human milk ranged from 0.670 to 7.83 ng/mL, with a mean value of 3.61 ng/mL. The highest mean concentration was found for tris-2-butoxyethyl phosphate (TBOEP, 1.44 ± 0.789 ng/mL), followed by tri-iso-butyl phosphate (TIBP, 0.569 ± 0.272 ng/mL) and tri-n-butyl phosphate (TNBP, 0.539 ± 0.265 ng/mL), which were the dominant OPEs found in breast milk at detection frequencies of >80%. No significant differences were observed between various maternal/infant characteristics and OPE concentrations (p > 0.05), except for TBOEP, for which the median concentrations in Hispanic mothers (0.765 ng/mL) were 2 times lower than those in non-Hispanic mothers (1.48 ng/mL) (p < 0.05). On the basis of the recommended daily milk ingestion rate, the average and the highest daily intakes of total OPEs were calculated to be in the range of 300-542 and 504-911 ng (kg of body weight)^-1 day^-1, respectively. The estimated daily intakes of OPEs did not exceed the current reference doses. Our study establishes baseline data for OPE exposure in breast-fed American children.

We determined the concentrations of melamine, ammeline, ammelide, and cyanuric acid in meat, fish and seafood, cereal products, beverages, cooking oil, and vegetables (n = 121) collected from Albany, New York, United States. In addition, food packaging (n = 24) and animal feed (n = 12) were analyzed to determine the sources of melamine and its derivatives in foods. Among the six categories of foods analyzed, median concentrations of ∑melamine (sum of melamine and its three derivatives) in meat (23.6 ng/g fresh weight; fw) and cereal products (20.9 ng/g fw) were significantly (p < 0.05) higher than those in other food categories (<5.03 ng/g fw). Cyanuric acid and melamine were the major compounds, accounting for 51% and 26% of the total ∑melamine concentrations, respectively. ∑melamine was found ubiquitously in food packaging (median: 36.2 ng/g fw) and animal feed (56.5 ng/g fw), which are two important sources of melamine found in foodstuffs. The median estimated daily dietary intakes (including concentrations reported for dairy products in our previous study) of melamine and cyanuric acid were in the ranges of 13.4-72.7 and 75.4-347 ng/kg body weight/day, respectively, for various age groups. Dairy products, cereal products, and meat were major sources of dietary melamine (~76%) and cyanuric acid (~95%) exposure. The calculated hazard quotients for dietary exposure to melamine and cyanuric acid were well below 1.0, which suggested minimal risk from current exposures.

Exposure of humans to pesticides is widespread. Measurement of urinary levels of pesticides and their metabolites is often used in the assessment of body burdens and exposure doses to these chemicals. An understanding of temporal variability in urinary levels of pesticides within individuals is critical for accurate exposure assessment. We examined within- and between-individual variability in concentrations of nine organophosphate and pyrethroid insecticides as well as two phenoxy herbicides in urine collected consecutively for up to 44 days from 19 individuals. Seven oxidative stress biomarkers also were measured in urine samples to elucidate their relationship with pesticide exposure. Intraclass correlation coefficients (ICCs) were calculated to assess reproducibility in urinary pesticide concentrations from repeated measures. Sensitivity and specificity analyses were performed to evaluate the suitability of spot urine to characterize average exposures. Data analysis was further limited to seven pesticides and their metabolites, which had a detection frequency of >60%. Poor reproducibility was found for the seven pesticides and their metabolites in both spot (ICCs ≤0.24) and first-morning-void (FMV) samples (ICCs <0.38) collected during the 44-day study period. Use of single-spot or FMV sample to classify high (top 33%) concentrations showed high specificities (0.73-0.85) but low sensitivities (0.45-0.70). The minimum number of samples (k) required per individual to estimate participant-specific mean value for pesticides (within 20% of the "true" values) were 28-140 and 18-119 for spot and FMV samples, respectively. Repeated longitudinal measurements of these pesticides and their metabolites in urine showed considerable within-individual variability in both spot and FMV samples. Urinary concentrations of seven pesticides and their metabolites were significantly correlated with oxidative damage to lipids, proteins, and DNA.