Searched for: in-biosketch:yes
person:saitom01
PEPTIDE MAPPING AND SEQUENCING OF THE CYTOSOL ENKEPHALIN-DEGRADING AMINOPEPTIDASE ISOZYMES FROM CHICKEN BRAIN
HUI K-S; SAITO M; HUI M; SAITO M; LAJTHA A
BIOSIS:PREV198937084640
ISSN: 0022-3042
CID: 115530
Lipid composition of PC12 pheochromocytoma cells: characterization of globoside as a major neutral glycolipid
Ariga T; Macala LJ; Saito M; Margolis RK; Greene LA; Margolis RU; Yu RK
We have studied the lipid composition of PC12 pheochromocytoma cells cultured in the presence and absence of nerve growth factor (NGF). Neutral and acidic lipid fractions were isolated by column chromatography on DEAE-Sephadex and analyzed by high-performance thin-layer chromatography (HPTLC). The total lipid concentration was approximately 220 micrograms/mg of protein, and the concentration of neutral glycolipids was 1.6-1.8 microgram/mg of protein for both NGF-treated and untreated cells. The neutral glycolipid fraction contained a major component, which accounted for approximately 80% of the total and which was characterized as globoside on the basis of HPTLC mobility, carbohydrate analysis, fast atom bombardment mass spectrometry, and mild acid hydrolysis. The major fatty acids of globoside were C16:0 (10%), C18:0 (16%), C22:0 (23%), C24:1 (17%), and C24:0 (24%). C18 sphingenine accounted for almost all of the long-chain bases. The other neutral glycolipids were tentatively identified as glucosylceramide (15%), lactosylceramide (4%), and globotriosylceramide (4.5%). The concentration of ganglioside sialic acid was approximately 0.34 and 0.18 microgram/mg of protein for cells grown in the presence and absence of NGF, respectively. Although there was an increase in ganglioside concentration in NGF-treated cells, NGF did not produce any differential effects on the relative proportions of the individual gangliosides. Several of the gangliosides appear to contain fucose, and one of these was tentatively identified as fucosyl-GM1. Brain-type gangliosides of the ganglio series were also detected by an HPTLC-immunostaining method. However, the fatty acid and long chain base compositions of PC12 cell gangliosides (and their TLC mobility) differ from those of brain gangliosides.(ABSTRACT TRUNCATED AT 250 WORDS)
PMID: 3349045
ISSN: 0006-2960
CID: 33490
The blockade of alpha 2-adrenoceptors by the PNMT inhibitaor SK&F 64139
Goldstein, M; Saito, M; Lew, J Y; Hieble, J P; Pendleton, R G
Three tetrahydroisoquinolines were tested for activity on central alpha 2-adrenoceptors by determining their ability to inhibit [3H]clonidine binding to rat cerebral cortical membrane homogenates. The compounds included the PNMT inhibitors SK&F 64139 (7,8-dichloro-1,2,3,4-tetrahydroisoquinoline) and SK&F 29661 (1,2,3,4-tetrahydroisoquinoline 7-sulfonamide) as well as SK&F 72223 (5,8-dimethoxy-1,2,3,4-tetrahydroisoquinoline) a structural analogue inactive as a PNMT inhibitor. SK&F 64139 and SK&F 72223 but not SK&F 29661 inhibited [3H]clonidine binding. Studies in the isolated guinea pig atrium confirmed that SK&F 64139 and SK&F 72223 are alpha 2-adrenoceptor antagonists
PMID: 6109637
ISSN: 0014-2999
CID: 147179
REDUCED NUMBER OF CARDIAC BETA-ADRENERGIC RECEPTORS IN RAT PHEOCHROMOCYTOMA [Meeting Abstract]
Shenkman, L; Saito, M; Feit, F; Goldstein, M
ISI:A1979HN21900219
ISSN: 0009-9279
CID: 30078
Reduced number of cardiac ?-adrenergic receptors in rat pheochromocytoma
Shenkman, L; Saito, M; Feit, F; Goldstein, M
SCOPUS:0018728326
ISSN: 0009-9279
CID: 580962