Faculty Bibliography

The direct analysis of 3D Optical Coherence Tomography (OCT) volumes enables deep learning models (DL) to learn spatial structural information and discover new bio-markers that are relevant to glaucoma. Downsampling 3D input volumes is the state-of-art solution to accommodate for the limited number of training volumes as well as the available computing resources. However, this limits the network's ability to learn from small retinal structures in OCT volumes. In this paper, our goal is to improve the performance by providing guidance to DL model during training in order to learn from finer ocular structures in 3D OCT volumes. Therefore, we propose an end-to-end attention guided 3D DL model for glaucoma detection and estimating visual function from retinal structures. The model consists of three pathways with the same network architecture but different inputs. One input is the original 3D-OCT cube and the other two are computed during training guided by the 3D gradient class activation heatmaps. Each pathway outputs the class-label and the whole model is trained concurrently to minimize the sum of losses from three pathways. The final output is obtained by fusing the predictions of the three pathways. Also, to explore the robustness and generalizability of the proposed model, we apply the model on a classification task for glaucoma detection as well as a regression task to estimate visual field index (VFI) (a value between 0 and 100). A 5-fold cross-validation with a total of 3782 and 10,370 OCT scans is used to train and evaluate the classification and regression models, respectively. The glaucoma detection model achieved an area under the curve (AUC) of 93.8% compared with 86.8% for a baseline model without the attention-guided component. The model also outperformed six different feature based machine learning approaches that use scanner computed measurements for training. Further, we also assessed the contribution of different retinal layers that are relevant to glaucoma. The VFI estimation model achieved a Pearson correlation and median absolute error of 0.75 and 3.6%, respectively, for a test set of size 3100 cubes.

Glaucoma is a neurodegenerative disease of the visual system and is the leading cause of irreversible blindness worldwide. To date, its pathophysiological mechanisms remain unclear. This study evaluated the feasibility of advanced diffusion magnetic resonance imaging techniques for examining the microstructural environment of the visual pathway in glaucoma. While conventional diffusion tensor imaging (DTI) showed lower fractional anisotropy and higher directional diffusivities in the optic tracts of glaucoma patients than healthy controls, diffusion kurtosis imaging (DKI) and the extended white matter tract integrity (WMTI) model indicated lower radial kurtosis, higher axial and radial diffusivities in the extra-axonal space, lower axonal water fraction, and lower tortuosity in the same regions in glaucoma patients. These findings suggest glial involvements apart from compromised axonal integrity in glaucoma. In addition, DKI and WMTI but not DTI parameters significantly correlated with clinical ophthalmic measures via optical coherence tomography and visual field perimetry testing. Taken together, DKI and WMTI provided sensitive and comprehensive imaging biomarkers for quantifying glaucomatous damage in the white matter tract across clinical severity complementary to DTI.

Purpose: Rigorous clinical testing has established that Schlemm"™s canal cross-sec-tional area (SC-CSA) is reduced in glaucomatous eyes. However, to date, it is unclear whether trabecular bypass procedures impact the morphology of the proximal aqueous outflow tract, or if the introduction of a local region of low outflow resistance adversely affects SC-CSA elsewhere, specifically presenting as SC diminution. This study quantifies changes in the morphology of the distal outflow pathway after iStent Trabecular Micro-Bypass stent (Glaukos Corp, Laguna Hills, CA, USA) implantation in living eyes by anterior segment optical coherence tomography (OCT). Design: This was a prospective observational study. Subjects: This study included six patients (eight eyes) with primary-open angle glaucoma. Methods: Patients underwent iStent placement in the nasal anterior chamber angle quadrant. OCT imaging was obtained of both nasal and temporal eye quadrants before and after surgery. For each SC parameter, an average of ten consecutive, evenly spaced measurements were manually obtained over a 1 mm segment of SC on FIJI ImageJ. Linear mixed effects modeling quantified the effect of the iStent on these parameters. Main outcome measures: Main outcome measures were changes in SC-CSA, inner-to-outer wall distance (IOD), and trabecular meshwork (TM) thickness following iStent placement. Results: Following iStent placement, total SC-CSA increased an average of 1,039.12 µm² (P = 0.05). Individually, there were no significant changes in SC-CSA in the nasal or temporal quadrants. Total SC-IOD and nasal SC-IOD increased an average of 2.35 µm (P = 0.01) and 2.96 µm (P = 0.04), respectively. There were no significant changes in temporal quadrant SC-IOD. There were no significant changes in TM thickness in either quadrant. Conclusions: Implantation of the iStent Trabecular Micro-Bypass stent significantly increases SC-IOD in the nasal quadrant at the location of implant, with no evidence of SC diminution in the temporal quadrant. It remains unclear how these observa-tions relate to the surgical efficacy of trabecular bypass procedures.

Purpose/UNASSIGNED:The purpose of this study was to develop a machine learning model to forecast future circumpapillary retinal nerve fiber layer (cpRNFL) thickness in eyes of healthy, glaucoma suspect, and glaucoma participants from multimodal temporal data. Design/UNASSIGNED:Retrospective analysis of a longitudinal clinical cohort. Participants/UNASSIGNED:Longitudinal clinical cohort of healthy, glaucoma suspect, and glaucoma participants. Methods/UNASSIGNED:(LTBE). Main Outcome Measures/UNASSIGNED:The mean absolute difference and Pearson's correlation coefficient between the true and forecasted values of the cpRNFL in the healthy, glaucoma suspect, and glaucoma patients. Results/UNASSIGNED:< 0.01) for the 3 groups, respectively. Conclusions/UNASSIGNED:The performance of the proposed forecasting model for cpRNFL is consistent across glaucoma suspect and glaucoma patients, which implies the robustness of the developed model against the disease state. These forecasted values may be useful to personalize patient care by determining the most appropriate intervisit schedule for timely interventions.

Purpose : The glymphatic system has been postulated to play a crucial role in the central nervous system via metabolic waste removal from brain tissues by the cerebrospinal fluid (CSF). However, it remains unclear whether there is a direct glymphatic pathway in the visual pathway, partly due to limited in vivo methods for assessing the physiology of CSF dynamics in the optic nerve (ON). Contrast-enhanced MRI has been shown to be capable of monitoring the dynamics of glymphatic system in the brain using paramagnetic contrast agents. Investigating the same in and around the ON might give insights into the mechanisms of vision-related diseases such as glaucoma. Methods : In the present study, we infused a small molecular weight gadolinium-DTPA contrast agent intrathecally into the lumbar region (L4-L5) of 3 healthy adult C57BL/6J mice and imaged its flow, accumulation and clearance in the brain and the optic nerve over time using a 7-Tesla MRI scanner under isoflurane anesthesia. Contrast dynamics was monitored using a 3D T1-weighted imaging sequence at an isotropic resolution of 78x78x78 mum . Each scan lasted 10 min and a total of 12 continuous scans were acquired. These scans included 3 baseline acquisitions followed by 30 min of gadolinium contrast infusion using an automated pump while the scanning continued until the 12th time point. The intensity-time curves of the ON parenchyma, ON subarachnoid space (SAS), olfactory bulb, lateral ventricles and muscle tissues were generated and compared quantitatively. Data are represented as mean+/-SEM. Results : The ON parenchyma, ON-SAS, olfactory bulb and lateral ventricles showed a gradual increase in contrast enhancement (Figures 1 and 2A) with peak intensities at 92.03+/-16.21% (p<0.05), 440.50+/-39.41% (p<0.01), 210.54+/-20.69% (p<0.01) and 196.63+/-38.63% (p<0.05) respectively relative to baseline (Figure 2B). Peak intensity 3 occurred first in the olfactory bulb followed by ON-SAS, ON parenchyma and finally the lateral ventricles (Figure 2B). No apparent contrast uptake was observed in the nearby muscle tissues. Conclusions : This study illustrates direct communications between CSF and ON parenchyma and supports the evidence of the glymphatic system in the ON. In vivo imaging of CSF dynamics in and around the ON may open up new avenues for understanding ON function in health and disease with the possibility of devising novel drug delivery routes and therapeutic targets

Purpose : Glaucoma is thought to involve neurochemical changes not only in the eye but also the brain's visual system. While excitotoxicity may play a role in glaucoma pathogenesis, it remains controversial whether excess glutamate occurs in this process. In the current study, we investigated alterations in the excitatory-inhibitory balance (E/I balance) in the visual cortex of glaucoma patients. In addition, we examined whether the altered neurochemical balance in the visual cortex is associated with projections of basal nucleus of Meynert (BNM), a major source of cortical cholinergic innervation in the basal forebrain. Methods : 10 glaucoma patients with a wide range of disease severity and 4 age-matched healthy subjects underwent 3-Tesla anatomical MRI, resting-state functional MRI (fMRI), and magnetic resonance spectroscopy (MRS). We used MEGA-PRESS and PRESS sequences to measure the levels of gamma-aminobutyric acid (GABA) and combined glutamate and glutamine (GLX), respectively. Both GABA and GLX were obtained from the same single voxel (2.2x2.2x2.2 cm³) placed along the calcarine sulci and fitted by LCModel software. We normalized the amount of GABA and GLX to N-acetyl-aspartate (NAA) values obtained from MEGA-PRESS, following LCModel guidelines. E/I balance was calculated by dividing the amount of GLX by the amount of GABA. The resting-state fMRI data were analyzed by CONN software. Results : Glaucoma patients had 16.51% higher E/I balance in the visual cortex compared to the healthy control group (Figure 1a). This difference in E/I balance was apparently driven by a 16.85% reduction in GABA (Figure 1b) with no apparent difference in glutamate or glutamine levels between groups (Figure 1c). Furthermore, the E/I balance in the visual cortex was correlated with the functional connectivity between BNM and the visual cortex (Figure 2). Conclusions : The current study shows that the visual cortex of glaucoma patients adopts an excitatory-dominant state that is driven by reduced GABA. This imbalance was associated with the functional connectivity between BNM and the visual cortex, suggesting that weaker projection of BNM to the visual cortex may play a role in the neurochemical changes in the visual cortex of glaucoma patients. Taken together, these findings suggest that widespread functional and metabolic alterations are involved in the brain during glaucoma pathogenesis

Purpose: The purpose of the present study was to quantify test-retest reproducibili-ty of measurements of stiffness of the human trabecular meshwork (HTM) by atomic force microscopy (AFM).
Method(s): Eleven 40 mum radial limbal cryostat sections from a fresh human donor rim were mounted on charged slides and rehydrated at room temperature. Stiffness at four TM locations (anterior to posterior along Schlemm's canal) was measured by AFM. At each location, a 6 x 6 grid was sampled. Indentation points were evenly distributed over a 20 mum x 20 mum area, with a rate of one load/unload cycle per second. Measurements were then repeated for calculation of test-retest variability.
Result(s): The test-retest coefficients of variation for the four measurement locations (anterior to posterior) were 24.39, 25.28, 12.74, and 14.26%, respectively, with a notable drop in the two posterior locations compared to the anterior. The test-retest coefficient for the sections was 19.17%. For the entire eye, the test-retest coefficient of variation for the measurement of the TM stiffness was 17.13%. Young's moduli consistently decreased from anterior to posterior location.
Conclusion(s): Wide regional variation suggests that single value does little to fully describe the complex array of TM stiffness levels within the eye, and future studies of TM stiffness assessed by AFM should include multiple tissue samples from each eye, with documentation of the anterior-posterior location of each measurement.
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