Faculty Bibliography

BACKGROUND: The rate of corneal epithelial wound healing may be determined, in part, by the characteristics of the stromal surface. The excimer laser has the ability to produce a highly uniform ablated surface, which may facilitate reepithelialization after photorefractive keratectomy (PRK). METHODS: The rate of corneal epithelial wound healing after excimer laser PRK was compared with the rate of reepithelialization after manual lamellar keratectomy. Ten rabbits received a 4-mm diameter ablation in one eye (fluence = 160 mJ/cm2) and a shallow, 5-mm diameter, manual lamellar keratectomy in the contralateral eye. At 0, 4, 8, 12, 24, 36, 48, 60, and 72 hours after wounding, sodium fluorescein was instilled, and photographs were taken, converted to video images, and digitized. Wound area was calculated for each time point and converted to wound radius; the slopes of the wound radius, plotted over time, were compared to determine rates of healing. Scanning electron microscopy was performed immediately after wounding to examine surface regularity. RESULTS: By 24 hours after wounding, corneas that had undergone PRK demonstrated a significantly faster rate of epithelial wound healing compared with eyes that underwent lamellar keratectomy (33.4 +/- 1.9 microns/hr vs 27.8 +/- 1.4 microns/hr, respectively, for 12 to 72 hours) (p < 0.0001). Scanning electron microscopy showed greater stromal surface irregularity in the corneas that had undergone lamellar keratectomy, compared with the laser-ablated corneas. CONCLUSIONS: This study demonstrates that the rate of epithelial wound healing is significantly faster after excimer laser PRK than after lamellar keratectomy in the rabbit. Variations in surface regularity and wound edge profile may contribute to differences in wound healing

BACKGROUND: The Autonomous Technologies T-PRK (Tracker-assisted Photorefractive Keratectomy) excimer laser system uses a small beam scanner that allows flexibility in the ablation pattern that is applied to the cornea and incorporates a sophisticated LADARVision eye tracker that is capable of following saccadic movements. This paper describes the first clinical results on sighted eyes for the correction of low myopia. METHODS: Forty-two normal sighted eyes of 42 patients were treated for spherical myopia between -1.00 diopters (D) and -6.00 D with 6 mm ablations. Visual acuity, refractive error, contrast sensitivity (with and without glare), corneal haze, endothelial cell density, and patient satisfaction were measured. RESULTS: Mean manifest refraction was -0.39 D +/- 0.68 D at 1 month with regression to -0.94 D at 3 months and -1.05 D at 6 months. At 6 months, 5 (20%) eyes were +/- 0.50 D and 14 (56%) eyes were +/- 1.00 D. Consistent with this undercorrection and regression, uncorrected visual acuity (UCVA) of 20/20 and 20/40 or better was achieved by 10 (40%) and 34 (85%) eyes at 3 months and 16 (40%) and 17 (68%) eyes at 6 months. None of the eyes lost 2 or more lines of spectacle corrected visual acuity. Corneal haze was graded as 1/2 trace or less in 89% to 100% of eyes at all intervals. There was no loss of endothelial cells (mean +/- SD cell density centrally: preop 3115 +/- 322 and 6 months 3220 +/- 333) and contrast sensitivity recovered to baseline levels at 3 months. CONCLUSIONS: The Autonomous Technologies T-PRK excimer laser system is safe and effective for the reduction or correction of myopia from -1.00 D or -6.00 D. The refractive results may be improved by adjusting the calibration to reduce the undercorrection and by instituting use of topical corticosteroids on an individual basis for those who regress

BACKGROUND: The theoretical benefits of synthetic keratophakia over conventional corneal lamellar procedures are the elimination of donor concerns and superior refractive predictability. Additionally, synthetic material can be inspected for optical quality and power, and it can be sterilized. Furthermore, visual recovery should be more rapid since epithelium is not removed from the central part of the cornea and the need for keratocyte repopulation is eliminated. OBJECTIVE: To present results on patients who received an intracorneal implant (Kerato-Gel, Allergan Medical Optics, Irvine, Calif) that was made from lidofilcon A, a glucose-permeable hydrogel with an equilibrium water content of 68%. METHODS: The intracorneal implants were implanted in 35 adult patients for correction of aphakia. Inclusion criteria excluded patients with aphakia who were candidates for intraocular lenses. RESULTS: A total of 19 patients were followed up through 2 years postoperatively. For 16 patients with 2-year postoperative refractive data, the average spherical equivalent was -0.63 +/- 2.07 diopters (D). At 2 years, 88% of patients were within +/- 3.00 D of plano and 50% were within +/- 1.00 D. the mean change in Snellen's line for corrected visual acuity was -3.25 lines at 2 years for all patients and -2.0 lines for a subgroup of five patients who were free of vision-limiting preoperative disease. CONCLUSIONS: Results suggest that this intracorneal implant is well tolerated by the cornea and can provide predictable refractive results in patients with high-risk aphakia. Limitations of the procedure are uneven microkeratome resections, loss of best-corrected visual acuity, and irregular astigmatism in some patients. Although these data show good evidence of biocompatibility of the implant material, technical surgical progress is needed to advance this procedure into clinical therapeutic practice

We used real-time scanning confocal microscopy to evaluate early changes in corneal wound healing after excimer laser photorefractive keratectomy (PRK). Adult New Zealand White rabbits were given photorefractive keratectomy treatments appropriate for 5.00 to 8.00 D of myopia (44.5 to 71.0 micros depth, with a 5-mm diameter treatment zone). Daily confocal microscopic examinations showed acute loss of keratocytes in the anterior corneal stroma by 5 hours; losses were maximal between 24 and 48 hours for 5.00 D and 6.00 D ablations and between 72 and 96 hours for 7.00 D and 8.00 D ablations. The oval nuclei of normal keratocytes gave way to spindle-shaped fibroblasts accompanied by an accumulation of fibrillary extracellular matrix. Fibroblasts density increased toward the end of the week. Deeper ablations resulted in a longer period of keratocyte depletion and delayed onset of fibroblast activity. No epithelial, deep stromal, or endothelial abnormalities were detected, nor was stromal inflammation found. Light microscopy 1 week after PRK revealed superficial fibroplasia, which correlated with the en face images obtained with real-time in vivo confocal microscopy. The confocal microscope has a number of advantages as a clinical tool for investigation of laser-induced changes in corneal keratocytes and the stromal matrix, which may play a role in determining visual outcome after PRK

BACKGROUND: Correction of residual myopia after radial keratotomy may be attempted with repeated keratotomy surgery, but predictability can be less than satisfactory. Excimer laser photorefractive keratectomy (PRK) provides an alternative approach to improving the refractive result in these patients. METHODS: Twenty-five eyes of 20 patients at five clinical locations underwent PRK for residual myopia after radial keratotomy. The number of incisions ranged from 4 to more than 16. Clear zones ranged from 3 mm to 4 mm. Best corrected visual acuity was 20/20 or better in 16 of the 25 eyes, with a range from 20/12 to 20/80. Uncorrected visual acuity was 20/200 or worse in 15 of the 25 eyes, with a range from 20/25 to finger counting. The interval between radial keratotomy and PRK averaged 33.5 months, with a range from 5 to 96 months. Nineteen eyes had 6 months or more of follow up; 15 had 12 months or more. RESULTS: Corneal haze was maximal 1 month after surgery (mean +/- SE, 0.65 +/- 0.09), and declined to 0.35 +/- 0.16 at 12 months. Twelve months after PRK, mean keratometric readings were 40.19 +/- 0.81 diopters (D) and mean spherical equivalent refraction was -1.42 +/- 0.47 D. Nine (60%) of the 15 eyes with 12 months follow up were within 1 D of emmetropia and 12 (80%) were within 2 D. Eight (53%) of the 15 eyes had uncorrected visual acuities of 20/40 or better. Spectacle-corrected visual acuity in the eyes with 12 months follow up improved in 4, did not change in 4, and worsened in 6. CONCLUSIONS: The results of PRK are less predictable in eyes that have previously undergone radial keratotomy, and these eyes respond with more haze after PRK than normal eyes

PURPOSE: In an investigational procedure, excimer laser photorefractive keratectomy for severe myopia was performed at three clinical trial centers to determine the effectiveness of the multiple zone technique. METHODS: A VisX Model Twenty/Twenty excimer laser (VisX, Santa Clara, California) was used to perform photorefractive keratectomy on 14 severely myopic eyes (-10.37 to -24.5 diopters) of 12 patients by using a multiple zone technique. Postoperative follow-up ranged from six months to two years; retreatments were performed on four patients, with a follow-up of at least nine months. RESULTS: At six months postoperatively, before retreatment, three of the 14 eyes were within 2 diopters and seven of the 14 eyes were within 4 diopters of attempted correction. Regression of effect to more severe myopia was worse in five eyes treated with nitrogen gas blowing. Retreatments also demonstrated considerable myopic regression. Three patients had loss of two or more lines of best-corrected visual acuity, and these patients also had moderate or severe levels of haze. CONCLUSION: Excimer laser photorefractive keratectomy for severe myopia using a multiple zone technique is associated with considerable regression, haze, and loss of best-corrected visual actuity, especially when performed in association with nitrogen gas blowing