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Dentin hypersensitivity, the biofilm and remineralization: what is the connection?

Wolff, M S
PMID: 19717406
ISSN: 1544-0737
CID: 154935

Pilot study: digital subtraction radiography as a tool to assess alveolar bone changes in periodontitis patients under treatment with subantimicrobial doses of doxycycline

Goren, Arthur D; Dunn, Stanley M; Wolff, Mark; van der Stelt, Paul F; Colosi, Dan C; Golub, Lorne M
BACKGROUND: Subtle changes in marginal alveolar bone level can be demonstrated using digital subtraction of sequential radiographs. OBJECTIVE: We aimed to evaluate the practical application of geometrically corrected digital subtraction in a clinical study of alveolar bone response to a drug previously shown to inhibit alveolar bone loss. STUDY DESIGN: Selected periapical radiographs were acquired with projective standardization of patients with clinical marginal alveolar bone loss. Subsequently, patients received a 6-month regimen of subantimicrobial doxycycline or placebo. Standardized radiographs of the same alveolar regions were acquired after 3 and 6 months, and baseline radiographs were subtracted from these images. RESULTS: Blinded digital subtraction analysis indicated various levels of marginal bone gain in 3 of the 6 patients in the experimental group, whereas in 3 of the 5 placebo patients the method showed bone loss. CONCLUSION: These results suggest that geometrically corrected digital subtraction possibly agrees with clinical predictors of bone loss severity
PMID: 18718780
ISSN: 1528-395x
CID: 153966

Characteristics of collagenase-2 from gingival crevicular fluid and peri-implant sulcular fluid in periodontitis and peri-implantitis patients: pilot study

Xu, Ling; Yu, Zhao; Lee, Hsi-Ming; Wolff, Mark S; Golub, Lorne M; Sorsa, Timo; Kuula, Heidi
OBJECTIVE: To compare collagenase activity and collagenolytic matrix metalloproteinase (MMP) levels in gingival crevicular fluid (GCF) and in peri-implant sulcular fluid (PISF) in gingivitis (G), chronic periodontitis (CP), and peri-implantitis (PI) human subjects. MATERIAL AND METHODS: GCF and PISF were collected on filter paper strips, volume was determined, and samples were extracted in buffer containing general proteinase but not MMP inhibitors. Collagenase activity was measured using a DNP-synthetic octapeptide, and molecular and activation forms of collagenase-2 by Western immunoblotting. RESULTS: GCF from CP and G sites exhibited elevated collagenase activity and flow, but collagenase concentrations expressed per microl were not significantly different between the healthy and G sites. Minimal fluid was obtained from healthy PISF, and collagenase concentration was the same or lower than in healthy GCF. Although PISF flow was 34% lower than GCF flow in CP subjects, collagenase concentration in CP and in PI sites was 78% and 971% greater, respectively, than in the appropriate healthy sites. Western immunoblot revealed MMP-8 in both PISF and GCF; fibroblast-type MMP-8 was not detected in healthy GCF and PISF. Immunoreactivity level and inactive and activated forms of PMN-type MMP-8 in GCF and PISF increased with the severity of periodontitis and peri-implantitis. Enhanced levels of fibroblast-type MMP-8 in active form were detected only in severe CP GCF and PI PISF. CONCLUSIONS: Peri-implantitis PISF contained higher collagenase-2 levels and activity than GCF from similar deep CP sites. GCF and PISF from severe CP and PI exhibited the highest activation of MMP-8 isoenzymes species (PMN and fibroblast-type)
PMID: 18615324
ISSN: 1502-3850
CID: 154690

Subantimicrobial-dose doxycycline modulates gingival crevicular fluid biomarkers of periodontitis in postmenopausal osteopenic women

Golub, Lorne M; Lee, Hsi Ming; Stoner, Julie A; Sorsa, Timo; Reinhardt, Richard A; Wolff, Mark S; Ryan, Maria E; Nummikoski, Pirkka V; Payne, Jeffrey B
BACKGROUND: We recently demonstrated that a 2-year subantimicrobial-dose doxycycline (SDD) regimen (double-masked, placebo-controlled clinical trial) in postmenopausal (PM) women exhibiting mild systemic bone loss (osteopenia) and local bone loss (periodontitis) reduced the progression of periodontal attachment loss (intent-to-treat analysis) and the severity of gingival inflammation and alveolar bone loss (subgroups) without producing antibiotic side effects. We now describe SDD effects on biomarkers of collagen degradation and bone resorption in the gingival crevicular fluid (GCF) of the same vulnerable subjects. METHODS: GCF was collected from SDD- and placebo-treated PM subjects (n=64 each) at the baseline and 1- and 2-year appointments; the volume was determined; and the samples were analyzed for collagenase activity (using a synthetic peptide as substrate), relative levels of three genetically distinct collagenases (Western blot), a type-1 collagen breakdown product/bone resorption marker (a carboxyterminal telopeptide cross-link fragment of type I collagen [ICTP]; radioimmunoassay), and interleukin-1beta (enzyme-linked immunosorbent assay). Statistical analyses were performed using generalized estimating equations; primary analyses were intent-to-treat. RESULTS: Collagenase activity was significantly reduced by SDD treatment relative to placebo based on intent-to-treat (P=0.01). ICTP showed a similar pattern of change during SDD treatment, and GCF collagenase activity and ICTP were positively correlated at all time periods (P<0.001). Matrix metalloproteinase (MMP)-8 accounted for approximately 80% of total collagenase in GCF, with much less MMP-1 and -13, and SDD reduced the odds of elevated MMP-8 by 60% compared to placebo (P=0.006). CONCLUSION: These observations support the therapeutic potential of long-term SDD therapy to reduce periodontal collagen breakdown and alveolar bone resorption in PM women; effects on serum biomarkers of systemic bone loss in these subjects are being analyzed
PMCID:2553351
PMID: 18672990
ISSN: 0022-3492
CID: 154689

Treatment planning in esthetic dentistry requires careful listening to the patient

Estafan, Denise; Klodnitskaya, Lisa; Wolff, Mark S
Creating an esthetic result that is pleasing to both the patient and the dentist requires careful consideration of patient concerns and treatment goals. This article presents a case involving a patient with many esthetic concerns. Following in-depth examination of the patient and consultations, an esthetic treatment plan involving the placement of laminate veneers was developed and executed. After the first set of laminate veneers was placed, some concerns led to an alteration in the proposed treatment plan. Each step of the treatment plan must be assessed before the next step is performed to guarantee a result that is both functional and esthetically pleasing
PMID: 19288840
ISSN: 0363-6771
CID: 154685

Effect of disinfection on bond strength to enamel and dentin

Corpodian C; Kaim J; Pines MS; Wolff MS
ORIGINAL:0007215
ISSN: 0022-0345
CID: 154824

In vitro effects of OTC bleaching on enamel and dentin bonding

Canares G; Pines M; Wolff MS
ORIGINAL:0007216
ISSN: 0022-0345
CID: 154825

Protecting root surfaces from demineralization utilizing dentin bonding

Morrison J; Wolff MS
ORIGINAL:0007217
ISSN: 0022-0345
CID: 154826

Overcoming barriers to implementing evidence-based dentistry

Spielman, Andrew I; Wolff, Mark S
PMID: 18316529
ISSN: 0022-0337
CID: 153022

Esthetics and orthodontics : breaking through discipline boundaries

Lipp MJ; Estafan DJ; Wolff MS; Cisneros G; Calamia J; Walla M
ORIGINAL:0007224
ISSN: 0022-0337
CID: 154833