Faculty Bibliography

Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [(125)I]S3 polysaccharide-protein conjugate. Binding of radioactivity in the gamma globulin region was observed. Extracts and media obtained from labeled cell cultures contained a protein that bound to an S3, but not to an S8 or inactivated S3 immunoadsorbent. After elution, the bound protein showed a single band on isoelectric focusing which corresponded in isoelectric point to one of the serum anti-S3 antibodies of the donor rabbit. These observations strongly suggest that a normal committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody.

While amylase secretion from both parotid and pancreas is mediated by cyclic AMP, only parotid secretion is influence by isoproterenol. Adenyl cyclase in both tissues is stimulated by isoproterenol, but there is much less enzyme activity in preparations from pancreas. After in vivo administration of isoproterenol, parotid cyclic AMP levels are greatly elevated whereas little change is observed in pancreas