Faculty Bibliography

The aim of this experiment was to identify the location of the biochemical changes associated with depressed mineralization during space flight. We carried out biochemical analysis of 4 sections of the femoral diaphyses from 107 day old male rats flown aboard Cosmos 2044 Biosatellite for 16 days. Control femurs were preflight, vivarium, synchronous for feed, cage and temperature exposure, and a flight simulation model. Distal sections in both the flight and synchronous femurs showed mineral deficits associated with reduced levels of the reducible cross-link product of type I collagen, dehydro-dihydroxylysinonorleucine (deH-DHLNL) (p<.05). Unloaded bones in the ground based flight simulation model showed changes in cross-links similar to flight and synchronous controls, but were not associated with the mineral deficit. Mean values of elements measured in each section of all groups revealed significant associations (p<.005) between the non-collagenous protein, osteocalcin and calcium (r=0.774), phosphorus (r=-.624) and deH-DHLNL/deH-HLNL (r=.883). The ratio of the nonreducible cross-link, pyridinoline, to its lysl analogue, deoxypyridinoline, was consistently lower in the distal than proximal sections of the groups tested. None of the changes during space flight were unique to flight bone. The most significant and extensive changes in bone composition, i.e. mineral deficits associated with changes in both osteocalcin and reducible cross-links, were located in the distal section of the diaphysis of the femur.

Detailed studies of biological phenomena often involve multiple microscopy and imaging modes and media. For bone biology, various forms of light and electron microscopy are used to study the microscopic structure of bone. Integrating information from the different sources is necessary to understand how different aspects of the bone structure interact. To accomplish this, methods were developed to prepare and image thin sections for correlative light microscopy (LM) and backscattered electron imaging in the scanning electron microscope (BSE-SEM). Images of the same fields of view may then be analyzed for degrees of relationships between specimen features not observed by LM or SEM alone. These methods are applied here to study possible associations between the degree of bone mineralization and pattern of collagen fiber orientation in the mid-shaft of the human femur. The 'relational images' obtained allow us to examine the relationship between these two variables, both objectively and quantitatively

Nicotine is one of the most widely used toxins in the world today. Most addiction research relating to nicotine in particular, as well as opioids and alcohol, has concentrated on the cellular and molecular biology of the mammalian brain and on features of organ structure and physiology associated with substance abuse. Thus, while numerous studies have been conducted to examine nicotine's detrimental physiological effects in a variety of soft tissues, this investigation attempts to examine further the gross morphological consequences of this drug on a hard tissue, the first molar crown of the laboratory rat. It is hypothesised that by providing nicotine to rats during and after the fetal cycle, changes in dental structure will occur, owing to perturbations of development induced by this toxin. The dentitions of Fisher rats exposed to nicotine during and after the fetal cycle, and those of their non-treated controls, were examined. By carefully measuring the length, width and occlusal (chewing) areas of the first maxillary molars, it was possible to identify any gross morphological effects of nicotine on dental development. It was found that dental asymmetries (calculated as a size difference between a tooth and its antimere) were significantly increased while occlusal areas were significantly decreased in nicotine-exposed rats compared to control rats. In addition, significant differences were detected within the experimental group, females tending to exhibit the deleterious effects of nicotine more so than males. These results are in accordance with the predicted outcome; in similar studies of physiological systems and soft tissues, dental development is affected by the presence of nicotine

An overview is given of image analytical procedures that utilize color rendering and graphic overlays to improve the visualization and quantification of data related to the research interest in bone histology. Emphasis is on the use of color because the human eye-brain complex is much more adept at morphological discrimination in color than in levels of grey.

A method for preparing nondecalcified bone and tooth specimens for imaging by both light microscopy (LM) and backscattered electron microscopy in the scanning electron microscope (BSE-SEM) is presented. Bone blocks are embedded in a polymethylmethacrylate (PMMA) mixture and mounted on glass slides using components of a light-cured dental adhesive system. This method of slide preparation allows correlative studies to be carried out between different microscopy modes, using the same histologic section. It also represents a large time savings relative to other mounting methods whose media require long cure times