Faculty Bibliography

The fine analysis of synaptic contacts is usually performed using transmission electron microscopy (TEM) and its combination with neuronal labeling techniques. However, the complex 3D architecture of neuronal samples calls for their reconstruction from serial sections. Here we show that focused ion beam/scanning electron microscopy (FIB/SEM) allows efficient, complete, and automatic 3D reconstruction of identified dendrites, including their spines and synapses, from GFP/DAB-labeled neurons, with a resolution comparable to that of TEM. We applied this technology to analyze the synaptogenesis of labeled adult-generated granule cells (GCs) in mice. 3D reconstruction of dendritic spines in GCs aged 3-4 and 8-9 weeks revealed two different stages of dendritic spine development and unexpected features of synapse formation, including vacant and branched dendritic spines and presynaptic terminals establishing synapses with up to 10 dendritic spines. Given the reliability, efficiency, and high resolution of FIB/SEM technology and the wide use of DAB in conventional EM, we consider FIB/SEM fundamental for the detailed characterization of identified synaptic contacts in neurons in a high-throughput manner.

ABSTRACT Minority children attending schools in urban socioeconomically disadvantaged neighborhoods are at high risk for conduct problems. Although a number of family and neighborhood characteristics have been implicated in the onset and progression of conduct problems, there remains incomplete understanding of the unique contributions of poverty-related factors early in development. This prospective study of 298 black public school children considered family- and neighborhood-level predictors of teacher-reported conduct problems from pre-kindergarten through first grade. Results from multi-level analyses indicate that percentage of poor residents in a student's neighborhood made a robust independent contribution to the prediction of development of conduct problems, over and above family- and other neighborhood-level demographic factors. For children of single parents, the percentage of black residents in the neighborhood also predicted the development of conduct problems. School-based interventions to prevent conduct problems should consider impact for children at highest risk based on neighborhood poverty.

This article provides an overview of the two books presented in the issue Journal of the American Academy of Child & Adolescent Psychiatry. The first book, Being a Teen: Everything Teen Girls and Boys Should Know About Relationships, Sex, Love, Health, Identity, and More By Jane Fonda is the culmination of the author's experience with teens and what the author believes teens need to know. The second book, Healing After Parent Loss in Childhood and Adolescence: Therapeutic Interventions and Theoretical Considerations edited by Phyllis Cohen, K. Mark Sossin, and Richard Ruth is a new volume dedicated to clinical work with children and adolescents who have lost a parent to death. Sickles reviews a book of advice for teens, and Weis reviews a new text describing therapeutic interventions for children after the loss of a parent.

BACKGROUND: Depression frequently co-occurs with diabetes. The associations between risk factors for insulin resistance and depression and diabetes can help determine the relative importance of factors that contribute toward the comorbidity. METHOD: Analyses of the NHANES I (n = 10,025) to examine the cross-sectional relationships between depression and risk factors for insulin resistance at baseline using logistic regression and to explore the longitudinal relationships between risk factors for insulin resistance and diabetes incidence using Cox proportional hazards modeling. RESULTS: Many risk factors for insulin resistance were associated with depression and diabetes incidence. Depression was cross-sectionally associated with diabetes, but did not increase the risk for diabetes incidence.These counterintuitive results can be explained primarily by the differing relationships between risk factors for insulin resistance, depression, and diabetes. LIMITATIONS: Lack of repeated measures of depression. CONCLUSIONS: Lack of physical activity, hypertension, and inadequate sleep were the risk factors for insulin resistance with the highest associations with both depression and diabetes incidence.

Reviews the book, Life, Animated: A Story of Sidekicks, Heroes, and Autism by Ron Suskind (2014). The book is not about autism and it is not even a story of a boy with autism. At times while experiencing Life, Animated, the reviewer thought that it was the story of a parent and family coming to understand and cope with a child with autism. The book, in fact, could be read with that idea in mind and still be an immensely rewarding experience for anyone who treats or works with children with autism and their families. In the end, however, the story is much more universal than that. It is the story of a father coming to know his son. It is the story of members of a family coming to know themselves. It is the story of a child's unique experience of his internal and external worlds. Beyond showing us the experience families have in dealing with difficult mental illness and disability or suggesting unique ways of engaging with children who might communicate in a different way, the book expands what one thinks of as the human experience. It suggests that experiencing life in a way that is considered more normative in the spectrum of human experience is not necessarily a better or right way to see things.

BACKGROUND: Borderline personality disorder (BPD) is a prevalent psychiatric disorder associated with significant distress, dysfunction, and treatment utilization. Though, theoretically, BPD is posited to arise from a combination of trait and environmental risk factors, few studies have tested trait-by-environment interactions in BPD. We investigated the roles of rejection sensitivity (RS) and childhood emotional neglect and abuse (ENA) as well as their interaction in BPD. SAMPLING AND METHODS: Eighty-five adults with a lifetime mood disorder who were recruited for outpatient studies in a psychiatric clinic were assessed for ENA using the Childhood Trauma Questionnaire and for RS with the Adult Rejection Sensitivity Questionnaire. BPD diagnoses were made by consensus using data collected on the Structured Clinical Interview for DSM-IV. Hierarchical logistic regression was used to test associations between RS, ENA, their interaction and BPD. RESULTS: RS and ENA interacted to predict co-occurring BPD in our sample of mood-disordered patients, with the strength of the relationship between RS and BPD depending on the severity of ENA. In the context of little or no ENA, RS and BPD were more strongly related than when ENA was more severe. CONCLUSIONS: Our results extend previous findings suggesting RS and ENA are risk factors for BPD. They also provide preliminary support for contemporary theories of BPD positing trait-by-environment interactions in the development of BPD. Prospective studies are needed to confirm these findings. (c) 2014 S. Karger AG, Basel.