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3D printed mesoporous bioactive glass, bioglass 45S5, and β-TCP scaffolds for regenerative medicine: A comparative in vitro study

Pacheco, Maria; Ricci, John L; Mijares, Dindo; Bromage, Timothy G; Rabieh, Sasan; Coelho, Paulo G; Witek, Lukasz
BACKGROUND:While autografts to date remain the "gold standard" for bone void fillers, synthetic bone grafts have garnered attention due to their advantages such as ability to be tailored in terms of its physical and chemical properties. Bioactive glass (BG), an inorganic material, has the capacity to form a strong bond with bone by forming a bone-like apatite surface, enhancing osteogenesis. Coupled with three-dimensional printing it is possible to maximize bone regenerative properties of the BG. OBJECTIVE:The objective of this study was to synthesize and characterize 3D printed mesoporous bioactive glass (MBG), BG 45S5, and compare to β-Tricalcium phosphate (β-TCP) based scaffolds; test cell viability and osteogenic differentiation on human osteoprogenitor cells in vitro. METHODS:MBG, BG 45S5, and β-TCP were fabricated into colloidal gel suspensions, tested with a rheometer, and manufactured into scaffolds using a 3D direct-write micro-printer. The materials were characterized in terms of microstructure and composition with Thermogravimetric Analyzer/Differential Scanning Calorimeter (TGA/DSC), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Micro-Computed Tomography (μ-CT), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), and Mattauch-Herzog-Inductively Coupled Plasma-Mass Spectrometry (MH-ICP-MS). RESULTS:Scaffolds were tested for cell proliferation and osteogenic differentiation using human osteoprogenitor cells. Osteogenic media was used for differentiation, and immunocytochemistry for osteogenic markers Runx-2, Collagen-I, and Osteocalcin. The cell viability results after 7 days of culture yielded significantly higher (p < 0.05) results in β-TCP scaffolds compared to BG 45S5 and MBG groups. CONCLUSION/CONCLUSIONS:All materials expressed osteogenic markers after 21 days of culture in expansion and osteogenic media.
PMID: 36744331
ISSN: 1878-3619
CID: 5434832

Life history in primate teeth is revealed by changes in major and minor element concentrations measured via field-emission SEM-EDS analysis

Cerrito, Paola; Hu, Bin; Kalisher, Rachel; Bailey, Shara E; Bromage, Timothy G
Overcoming the non-specificity of histological accentuated growth lines in hard tissues is an ongoing challenge. Identifying season at death and reproductive events has profound implications for evolutionary, ecological and conservation studies. Dental cementum is a mineralized tissue with yearly periodicity that continues deposition from tooth formation until death, maintaining a record spanning almost the entire life of an individual. Recent work has successfully employed elemental analysis of calcified incremental tissues to detect changes in extrinsic conditions such as diet and climate and to identify two important life-history milestones: weaning and sexual maturity. Here, we employ field-emission scanning electron microscopy and energy-dispersive X-ray analysis to measure the relative concentrations of calcium, phosphorous, oxygen, magnesium and sodium in the cementum of 34 teeth from seven male and female rhesus macaques with known medical and life-history information. We find that changes in relative magnesium concentrations correspond with reproductive events in females and breastfeeding in infants. Additionally, we observe seasonal calcium patterns in 77.3% of the samples.
PMID: 36651149
ISSN: 1744-957x
CID: 5410722

Dental cementum virtual histology of Neanderthal teeth from Krapina (Croatia, 130-120 kyr): an informed estimate of age, sex and adult stressors

Cerrito, Paola; Nava, Alessia; Radovčić, Davorka; Borić, Dušan; Cerrito, Leonardo; Basdeo, Tricia; Ruggiero, Guido; Frayer, David W; Kao, Alexander P; Bondioli, Luca; Mancini, Lucia; Bromage, Timothy G
The evolution of modern human reproductive scheduling is an aspect of our life history that remains vastly uncomprehended. The present work aims to address this gap by validating a non-destructive cutting-edge methodology to infer adult life-history events on modern teeth with known life history and then applying it to fossil specimens. We use phase-contrast synchrotron X-ray microtomography to visualize the dental cementum of 21 specimens: nine contemporary humans; 10 Neanderthals from Krapina (Croatia, 130-120 kyr); one Neolithic Homo sapiens from Ajmana (Serbia); and one Mesolithic H. sapiens from Vlasac (Serbia). We were able to correctly detect and time (root mean square error = 2.1 years; R
PMID: 35193386
ISSN: 1742-5662
CID: 5172142

Ontogenetic variability in collagen fiber orientation at the midshaft femur of Virunga mountain gorillas (Gorilla beringei beringei) [Meeting Abstract]

Batsche, Kailie M.; Mcfarlin, Shannon C.; Arbenz-Smith, Keely; Gilardi, Kirsten; Muhire, Thadee; Stoinski, Tara S.; Mudakikwa, Antoine; Muvunyi, Richard; Hu, Bin; Bromage, Timothy G.
ISSN: 2692-7691
CID: 5207782

Elemental composition of primary lamellar bone differs between parous and nulliparous rhesus macaque females

Cerrito, Paola; Hu, Bin; Goldstein, Justin Z; Kalisher, Rachel; Bailey, Shara E; Bromage, Timothy G
Extracting life history information from mineralized hard tissues of extant and extinct species is an ongoing challenge in evolutionary and conservation studies. Primary lamellar bone is a mineralized tissue with multidien periodicity that begins deposition prenatally and continues until adulthood albeit with concurrent resorption, thus maintaining a record spanning several years of an individual's life. Here, we use field-emission scanning electron microscopy and energy-dispersive X-ray analysis to measure the relative concentrations of calcium, phosphorous, oxygen, magnesium and sodium in the femora of seven rhesus macaque with known medical and life-history information. We find that the concentration of these elements distinguishes parous from nulliparous females; that in females calcium and phosphorus are lower in bone formed during reproductive events; and that significant differences in relative magnesium concentration correlate with breastfeeding in infants.
PMID: 36318529
ISSN: 1932-6203
CID: 5365332

Optimizing Preparation Protocols and Microscopy for Cementochronology

Chapter by: Cerrito, Paola; Naji, Stephan; Bromage, Timothy
in: Dental Cementum In Anthropology by
pp. 189-200
ISBN: 978-1-108-47708-6
CID: 5441252

Adjuvant therapy with 1% alendronate gel for experimental periodontitis treatment in rats

Kajimoto, Natália de Campos; de Paiva Buischi, Yvonne; Loomer, Peter Michael; Bromage, Timothy G; Ervolino, Edilson; Fucini, Stephen Enrico; Pola, Natália Marcumini; Pirovani, Beatriz Ommati; Morábito, Maria Juliana Sismeiro; de Almeida, Juliano Milanezi; Chaves Furlaneto, Flávia Aparecida; Nagata, Maria José Hitomi
PURPOSE/OBJECTIVE:The aim of this study was to evaluate the effects of locally delivered 1% alendronate (ALN) gel used as an adjunct to non-invasive periodontal therapy. METHODS:0.05. RESULTS:>0.05 for all). CONCLUSIONS:Locally delivered 1% ALN gel used as an adjunct to SRP enhanced bone regeneration in the furcation region in a rat model of experimental periodontitis.
PMID: 34965617
ISSN: 2093-2278
CID: 5110772

Microbial contamination survey of environmental fresh and saltwater resources of upolu island, Samoa

Ochsenkühn, Michael A.; Fei, Cong; Bayaara, Odmaa; Romeo, Emarosa; Amosa, Patila; Idaghdour, Youssef; Goldstein, Gary; Bromage, Timothy G.; Amin, Shady A.
Analysis of microbe diversity in freshwater resources and nearshore seawater samples of Upolu Island was performed to investigate the distribution of harmful bacteria. For this, 124 samples were collected from 23 river systems, two volcanic lakes, and 45 locations inside and outside the barrier reef of Upolu Island, Samoa. Physicochemical parameters for general water quality, detection of coliform bacteria and 16S rRNA amplicon sequencing were performed on all samples. Fecal indicator bacteria (FIB) testing indicated a wide distribution of coliform bacteria in all sampled freshwater sites with evidence of fecal coliform in most locations. Importantly, evidence of coliform bacteria was found in most seawater samples inside and along the reef, apart from those samples taken ~20 km offshore. Illumina 16S rRNA amplicon sequencing of the V4 hypervariable region confirmed the presence of various types of harmful bacterial species, namely from the Enterobacteriaceae, Enterococcae, Streptococcaceae, and Vibrionacea families. By combining the sensitivity of FIB testing and next-generation sequencing, we were able to show the extent of potential contaminations in fresh and seawater samples and simultaneously identify the potential pathogenic bacterial genera present. The wide distribution of potential harmful bacteria from river runoff or direct sewage dumping has an impact on human health, leading to many skin and intestinal diseases, and is potentially detrimental to coral reef community health.
ISSN: 2076-3298
CID: 5057362

Adaptive Immune Response Associated with a Zirconium-Containing, Cemented, Total Knee Arthroplasty: A Case Report [Case Report]

Tarity, T David; Vigdorchik, Jonathan M; Westrich, Geoffrey H; Gonzalez Della Valle, Alejandro; Cerrito, Paola; Baral, Elexis C; Bromage, Timothy G; Bauer, Thomas W
CASE:A 69-year-old woman underwent revision total knee arthroplasty for patellar component aseptic loosening. The periprosthetic tissue demonstrated histologic features of an adaptive immune response (aseptic lymphocyte-dominant vasculitis-associated lesion [ALVAL]). No particles of corrosion debris were identified. The inflammation seemed to be associated with zirconium oxide (ZrO2) particles added as a bone cement radio-opacifier. CONCLUSION:The factors responsible for the adaptive immune response cannot be determined with certainty; however, this is the first reported case of ALVAL associated with ZrO2-containing bone cement. Previous reports describing ALVAL around failed total knee prostheses have not included observations about the type of contrast material added to cement.
PMID: 34449449
ISSN: 2160-3251
CID: 5200082

The oronasopharyngeal space and renewed formalization of the functional matrix hypothesis [Editorial]

Bromage, Timothy G
PMID: 34264168
ISSN: 2151-0903
CID: 4938812